MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
3.6.4.10	Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement; non-chaperonin molecular chaperone ATPase

Structure

PDB	Resolution (Å)	PDB name
3IUC	2.4	Crystal structure of the human 70kDa heat shock protein 5 (BiP/GRP78) ATPase domain in complex with ADP
3LDL	2.3	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with ATP
3LDN	2.2	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in apo form
3LDO	1.95	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with AMPPNP
3LDP	2.2	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with small molecule inhibitor
5E84	2.99	ATP-bound state of BiP
5E85	2.57	isolated SBD of BiP
5E86	2.681	isolated SBD of BiP with loop34 modification
5EVZ	1.85	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with ADP and inorganic phosphate
5EX5	1.9	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with 7-deaza-ADP and inorganic phosphate
5EXW	1.9	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with 7-deaza-ATP
5EY4	1.86	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with 2'-deoxy-ATP
5F0X	1.6	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with 2'-deoxy-ADP and inorganic phosphate
5F1X	1.9	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with ATP
5F2R	2.15	Crystal structure of human GRP78 (70kDa heat shock protein 5 / BIP) ATPase domain in complex with AMP-PCP
6ASY	1.85	BiP-ATP2
6CZ1	1.68	Crystal structure of ATPase domain of Human GRP78 bound to Ver155008
6DFM	2.14	Crystal structure of human GRP78 in complex with 8-aminoadenosine
6DFO	2.54	Crystal structure of human GRP78 in complex with 8-bromoadenosine
6DO2	1.7	Crystal structure of human GRP78 in complex with 7-deaza-2'-C-methyladenosine
6DWS	1.9	Crystal structure of human GRP78 in complex with (2R,3R,4S,5R)-2-(6-amino-8-((2-chlorobenzyl)amino)-9H-purin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
6ZMD	2.64	Crystal structure of HYPE covalently tethered to BiP bound to AMP-PNP
7N1R	2.03	A novel and unique ATP hydrolysis to AMP by a human Hsp70 BiP

GO term

GO ontology	GO term	GO description
Biological Process	GO:0006983	ER overload response
Biological Process	GO:0071236	cellular response to antibiotic
Biological Process	GO:0071320	cellular response to cAMP
Biological Process	GO:0071277	cellular response to calcium ion
Biological Process	GO:0071480	cellular response to gamma radiation
Biological Process	GO:0042149	cellular response to glucose starvation
Biological Process	GO:0071353	cellular response to interleukin-4
Biological Process	GO:0071287	cellular response to manganese ion
Biological Process	GO:1990090	cellular response to nerve growth factor stimulus
Biological Process	GO:0071466	cellular response to xenobiotic stimulus
Biological Process	GO:0021680	cerebellar Purkinje cell layer development
Biological Process	GO:0021589	cerebellum structural organization
Biological Process	GO:0051085	chaperone cofactor-dependent protein refolding
Biological Process	GO:0030968	endoplasmic reticulum unfolded protein response
Biological Process	GO:0001554	luteolysis
Biological Process	GO:0035437	maintenance of protein localization in endoplasmic reticulum
Biological Process	GO:1903895	negative regulation of IRE1-mediated unfolded protein response
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0031333	negative regulation of protein-containing complex assembly
Biological Process	GO:0030512	negative regulation of transforming growth factor beta receptor signaling pathway
Biological Process	GO:0051402	neuron apoptotic process
Biological Process	GO:0030182	neuron differentiation
Biological Process	GO:0030335	positive regulation of cell migration
Biological Process	GO:0010976	positive regulation of neuron projection development
Biological Process	GO:0031398	positive regulation of protein ubiquitination
Biological Process	GO:1990440	positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
Biological Process	GO:0031204	post-translational protein targeting to membrane, translocation
Biological Process	GO:0034975	protein folding in endoplasmic reticulum
Biological Process	GO:0042026	protein refolding
Biological Process	GO:1903891	regulation of ATF6-mediated unfolded protein response
Biological Process	GO:1903894	regulation of IRE1-mediated unfolded protein response
Biological Process	GO:1903897	regulation of PERK-mediated unfolded protein response
Biological Process	GO:0060904	regulation of protein folding in endoplasmic reticulum
Biological Process	GO:0042220	response to cocaine
Biological Process	GO:1904313	response to methamphetamine hydrochloride
Biological Process	GO:0097501	stress response to metal ion
Biological Process	GO:0021762	substantia nigra development
Biological Process	GO:1901998	toxin transport
Biological Process	GO:0030433	ubiquitin-dependent ERAD pathway
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0140662	ATP-dependent protein folding chaperone
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0031072	heat shock protein binding
Molecular Function	GO:0051787	misfolded protein binding
Molecular Function	GO:0019904	protein domain specific binding
Molecular Function	GO:0044183	protein folding chaperone
Molecular Function	GO:0051087	protein-folding chaperone binding
Molecular Function	GO:0043022	ribosome binding
Molecular Function	GO:0031625	ubiquitin protein ligase binding
Molecular Function	GO:0051082	unfolded protein binding
Cellular Component	GO:0009986	cell surface
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0034663	endoplasmic reticulum chaperone complex
Cellular Component	GO:0005788	endoplasmic reticulum lumen
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0005793	endoplasmic reticulum-Golgi intermediate compartment
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005925	focal adhesion
Cellular Component	GO:0043231	intracellular membrane-bounded organelle
Cellular Component	GO:0042470	melanosome
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0030496	midbody
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0005790	smooth endoplasmic reticulum

InterPro / Pfam

InterPro	InterPro name
IPR013126	Heat shock protein 70 family
IPR018181	Heat shock protein 70, conserved site
IPR029047	Heat shock protein 70kD, peptide-binding domain superfamily
IPR029048	Heat shock protein 70kD, C-terminal domain superfamily
IPR042050	Endoplasmic reticulum chaperone BIP, nucleotide-binding domain
IPR043129	ATPase, nucleotide binding domain

Pfam	Pfam name
PF00012	Hsp70 protein

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-114608	Platelet degranulation	Leaf	R-HSA-109582	Hemostasis
R-HSA-3371453	Regulation of HSF1-mediated heat shock response	Leaf	R-HSA-8953897	Cellular responses to stimuli
R-HSA-381033	ATF6 (ATF6-alpha) activates chaperones	Internal node	R-HSA-8953897	Cellular responses to stimuli
R-HSA-381042	PERK regulates gene expression	Internal node	R-HSA-8953897	Cellular responses to stimuli
R-HSA-381070	IRE1alpha activates chaperones	Internal node	R-HSA-8953897	Cellular responses to stimuli
R-HSA-381183	ATF6 (ATF6-alpha) activates chaperone genes	Leaf	R-HSA-8953897	Cellular responses to stimuli
R-HSA-983170	Antigen Presentation: Folding, assembly and peptide loading of class I MHC	Leaf	R-HSA-168256	Immune System

Subcellular location

Location	ECO term	Pubmed
Cell surface	ECO:0000269	PubMed:15098107
Cell surface	ECO:0000269	PubMed:20484814
Cell surface	ECO:0000269	PubMed:24355926
Cell surface	ECO:0000269	PubMed:27159390
Cell surface	ECO:0000269	PubMed:28053106
Cell surface	ECO:0000269	PubMed:33432092
Cytoplasm	ECO:0000250
Endoplasmic reticulum lumen	ECO:0000269	PubMed:21080038
Endoplasmic reticulum lumen	ECO:0000269	PubMed:21289099
Endoplasmic reticulum lumen	ECO:0000269	PubMed:23990668
Endoplasmic reticulum lumen	ECO:0000269	PubMed:29497057
Melanosome	ECO:0000269	PubMed:12643545

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adipose tissue	adipocytes	Medium	Enhanced
adrenal gland	glandular cells	Medium	Enhanced
appendix	endocrine cells	Medium	Enhanced
appendix	enterocytes	Medium	Enhanced
appendix	germinal center cells	Medium	Enhanced
appendix	goblet cells	Medium	Enhanced
appendix	non-germinal center cells	Medium	Enhanced
bone marrow	hematopoietic cells	Medium	Enhanced
breast	adipocytes	Low	Enhanced
breast	glandular cells	Medium	Enhanced
breast	myoepithelial cells	Low	Enhanced
bronchus	respiratory epithelial cells	Medium	Enhanced
caudate	glial cells	Medium	Enhanced
caudate	neuronal cells	High	Enhanced
cerebellum	Purkinje cells	Medium	Enhanced
cerebellum	cells in granular layer	Low	Enhanced
cerebellum	cells in molecular layer	High	Enhanced
cerebral cortex	endothelial cells	Low	Enhanced
cerebral cortex	glial cells	Medium	Enhanced
cerebral cortex	neuronal cells	High	Enhanced
cervix	glandular cells	Low	Enhanced
cervix	squamous epithelial cells	Low	Enhanced
colon	endocrine cells	High	Enhanced
colon	endothelial cells	Medium	Enhanced
colon	enterocytes	High	Enhanced
colon	goblet cells	High	Enhanced
colon	mucosal lymphoid cells	High	Enhanced
duodenum	endocrine cells	Medium	Enhanced
duodenum	enterocytes	High	Enhanced
duodenum	glands of Brunner	Medium	Enhanced
duodenum	goblet cells	Medium	Enhanced
duodenum	paneth cells	Medium	Enhanced
endometrium 1	cells in endometrial stroma	Low	Enhanced
endometrium 1	glandular cells	High	Enhanced
endometrium 2	cells in endometrial stroma	Medium	Enhanced
endometrium 2	glandular cells	High	Enhanced
epididymis	glandular cells	Medium	Enhanced
esophagus	squamous epithelial cells	Low	Enhanced
fallopian tube	glandular cells	Medium	Enhanced
gallbladder	glandular cells	Medium	Enhanced
heart muscle	cardiomyocytes	Medium	Enhanced
hippocampus	glial cells	Low	Enhanced
hippocampus	neuronal cells	High	Enhanced
kidney	cells in glomeruli	Low	Enhanced
kidney	cells in tubules	Medium	Enhanced
liver	cholangiocytes	Medium	Enhanced
liver	hepatocytes	Medium	Enhanced
lung	alveolar cells type II	High	Enhanced
lung	macrophages	High	Enhanced
lymph node	germinal center cells	Medium	Enhanced
lymph node	non-germinal center cells	Medium	Enhanced
nasopharynx	respiratory epithelial cells	Medium	Enhanced
oral mucosa	squamous epithelial cells	Low	Enhanced
ovary	follicle cells	Low	Enhanced
ovary	ovarian stroma cells	Medium	Enhanced
pancreas	exocrine glandular cells	Medium	Enhanced
pancreas	pancreatic endocrine cells	Low	Enhanced
parathyroid gland	glandular cells	Medium	Enhanced
placenta	cytotrophoblasts	Medium	Enhanced
placenta	decidual cells	High	Enhanced
placenta	endothelial cells	High	Enhanced
placenta	hofbauer cells	High	Enhanced
placenta	syncytiotrophoblasts - cell body	High	Enhanced
placenta	syncytiotrophoblasts - microvilli	High	Enhanced
prostate	glandular cells	Medium	Enhanced
rectum	endocrine cells	High	Enhanced
rectum	endothelial cells	Medium	Enhanced
rectum	enterocytes	High	Enhanced
rectum	fibroblasts	Medium	Enhanced
rectum	goblet cells	High	Enhanced
rectum	mucosal lymphoid cells	High	Enhanced
rectum	peripheral nerve/ganglion	Medium	Enhanced
salivary gland	glandular cells	Medium	Enhanced
seminal vesicle	glandular cells	Medium	Enhanced
skeletal muscle	myocytes	Medium	Enhanced
skin 1	cells in basal layer	High	Enhanced
skin 1	cells in granular layer	Medium	Enhanced
skin 1	cells in spinous layer	Medium	Enhanced
skin 1	eccrine glands	Low	Enhanced
skin 1	endothelial cells	High	Enhanced
skin 1	fibrohistiocytic cells	High	Enhanced
skin 1	langerhans cells	Medium	Enhanced
skin 1	lymphocytes	High	Enhanced
skin 1	melanocytes	High	Enhanced
skin 2	cells in basal layer	High	Enhanced
skin 2	cells in granular layer	Medium	Enhanced
skin 2	cells in spinous layer	Medium	Enhanced
skin 2	endothelial cells	Medium	Enhanced
skin 2	fibrohistiocytic cells	High	Enhanced
skin 2	langerhans cells	Medium	Enhanced
skin 2	lymphocytes	High	Enhanced
skin 2	melanocytes	High	Enhanced
small intestine	endocrine cells	High	Enhanced
small intestine	enterocytes	High	Enhanced
small intestine	goblet cells	High	Enhanced
small intestine	paneth cells	High	Enhanced
smooth muscle	smooth muscle cells	Medium	Enhanced
soft tissue 1	chondrocytes	Medium	Enhanced
soft tissue 1	fibroblasts	Low	Enhanced
soft tissue 1	peripheral nerve	Low	Enhanced
soft tissue 2	chondrocytes	Medium	Enhanced
soft tissue 2	fibroblasts	Medium	Enhanced
soft tissue 2	peripheral nerve	Low	Enhanced
spleen	cells in red pulp	Low	Enhanced
spleen	cells in white pulp	Low	Enhanced
stomach 1	glandular cells	Medium	Enhanced
stomach 2	glandular cells	Medium	Enhanced
testis	Leydig cells	Medium	Enhanced
testis	elongated or late spermatids	Medium	Enhanced
testis	pachytene spermatocytes	Low	Enhanced
testis	round or early spermatids	Low	Enhanced
testis	sertoli cells	Low	Enhanced
thyroid gland	glandular cells	High	Enhanced
tonsil	germinal center cells	Low	Enhanced
tonsil	non-germinal center cells	Medium	Enhanced
tonsil	squamous epithelial cells	Medium	Enhanced
urinary bladder	urothelial cells	Medium	Enhanced
vagina	squamous epithelial cells	Low	Enhanced

Summary of P11021

Gene: HSPA5, GRP78
Protein: Endoplasmic reticulum chaperone BiP

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P11021

Gene: HSPA5, GRP78 Protein: Endoplasmic reticulum chaperone BiP

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: HSPA5, GRP78
Protein: Endoplasmic reticulum chaperone BiP