MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
5.3.2.1	Isomerases; Intramolecular oxidoreductases; Interconverting keto- and enol-groups; phenylpyruvate tautomerase
5.3.3.12	Isomerases; Intramolecular oxidoreductases; Transposing C=C bonds; L-dopachrome isomerase

Structure

PDB	Resolution (Å)	PDB name
1CA7	2.5	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH HYDROXPHENYLPYRUVATE
1CGQ	2.0	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH ALANINE INSERTED BETWEEN PRO-1 AND MET-2
1GCZ	1.9	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) COMPLEXED WITH INHIBITOR.
1GD0	1.5	HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
1GIF	1.9	HUMAN GLYCOSYLATION-INHIBITING FACTOR
1LJT	2.0	Crystal Structure of Macrophage Migration Inhibitory Factor complexed with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole-acetic acid methyl ester (ISO-1)
1MIF	2.6	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
1P1G	2.5	MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH PRO-1 MUTATED TO GLY-1
2OOH	1.85	Crystal Structure of MIF bound to a Novel Inhibitor, OXIM-11
2OOW	1.75	MIF Bound to a Fluorinated OXIM Derivative
2OOZ	1.8	Macrophage Migration Inhibitory Factor (MIF) Complexed with OXIM6 (an OXIM Derivative Not Containing a Ring in its R-group)
3B9S	1.8	Macrophage Migration Inhibitory Factor (MIF) complexed with Inhibitor, 4-IPP.
3CE4	1.55	Structure of Macrophage Migration Inhibitory Factor Covalently Inhibited by PMSF Treatment
3DJH	1.25	Macrophage Migration Inhibitory Factor (MIF) at 1.25 A Resolution
3DJI	1.95	Crystal Structure of Macrophage Migration Inhibitory Factor Bound to an Acetaminophen Dimer Derived from NAPQI
3HOF	1.9	Structure of macrophage migration inhibitory factor (MIF) with caffeic acid at 1.9A resolution
3IJG	1.7	Macrophage Migration Inhibitory Factor (MIF) Bound to the (R)-Stereoisomer of AV1013
3IJJ	1.25	Ternary Complex of Macrophage Migration Inhibitory Factor (MIF) Bound Both to 4-hydroxyphenylpyruvate and to the Allosteric Inhibitor AV1013 (R-stereoisomer)
3JSF	1.93	Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 638 at 1.93a resolution
3JSG	1.58	Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 707 at 1.58a resolution
3JTU	1.86	Crystal structure of macrophage migration inhibitory factor (mif) with hydroxyquinoline inhibitor 708 at 1.86a resolution
3L5P	1.8	Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyridazinol inhibitor at 1.80A resolution
3L5R	1.94	Crystal structure of macrophage migration inhibitory factor (MIF) with phenylchromenone inhibitor at 1.94A resolution
3L5S	1.86	Crystal structure of macrophage migration inhibitory factor (MIF) with imidazopyrimidinylphenyl inhibitor at 1.86A resolution
3L5T	1.86	Crystal structure of macrophage migration inhibitory factor (MIF) with thiophenepiperazinylquinolinone inhibitor at 1.86A resolution
3L5U	1.9	Crystal structure of macrophage migration inhibitory factor (MIF) with benzothiazole inhibitor at 1.90A resolution
3L5V	1.7	Crystal structure of macrophage migration inhibitory factor (MIF) with glycerol at 1.70A resolution
3SMB	1.6	Phenethylisothiocyanate Covalently Bound to Macrophage Migration Inhibitory Factor (MIF)
3SMC	1.8	Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane
3U18	1.9	Chicago Sky Blue 6B, A Novel Inhibitor for Macrophage Migration Inhibitory Factor
3WNR	2.008	Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor
3WNS	1.658	Allyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor
3WNT	2.074	Multiple binding modes of benzyl isothiocyanate inhibitor complexed with Macrophage Migration Inhibitory Factor
4ETG	1.61	Crystal Structure of MIF L46G mutant
4EUI	1.7	Crystal Structure of MIF L46F mutant
4EVG	1.7	Crystal Structure of MIF L46A mutant
4F2K	1.53	Macrophage Migration Inhibitory Factor covalently complexed with phenethylisothiocyanate
4GRN	1.25	crystal structure of PAAM mutant of human MIF
4GRO	2.0	Crystallographic and biological characterization of N- and C- terminus mutants of human MIF
4GRP	1.27	Crystallographic and biological characterization of N- and C- terminus mutants of human MIF
4GRQ	1.65	Characterization of N- and C- terminus mutants of human MIF
4GRR	1.47	characterization of N- and C- terminus mutants of human MIF
4GRU	1.92	crystallographic and biological characterization of N- and C- terminus mutants of human MIF
4GUM	2.33	Cystal structure of locked-trimer of human MIF
4K9G	1.55	1.55 A Crystal Structure of Macrophage Migration Inhibitory Factor bound to ISO-66 and a related compound
4OSF	1.62	4-(2-isothiocyanatoethyl)phenol inhibitor complexed with Macrophage Migration Inhibitory Factor
4OYQ	1.7	(6-isothiocyanatohexyl)benzene inhibitor complexed with Macrophage Migration Inhibitory Factor
4P01	2.07	Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with N-[(4-cyanophenyl)methyl]methanethioamide
4P0H	1.93	Crystal Structure Analysis of Macrophage Migration Inhibitory Factor in complex with Dimethylformamide
4PKK	1.78	Crystal structure of Macrophage Migration inhibitory factor in complex with furan-2-ylmethyl)imino methanethiol
4PKZ	1.9	Crystal structure of P1M mutant of Macrophage Migration Inhibitory Factor
4PLU	1.63	Crystal structure of Macrophage Migration Inhibitory Factor in complex with benzaldehyde
4TRF	1.63	Crystal structure of Macrophage Migration Inhibitory Factor in complex with N-(pyridin-3-ylmethyl)thioformamide
4TRU	1.81	Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor
4WR8	2.6	Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-180)
4WRB	1.81	Macrophage Migration Inhibitory Factor in complex with a biaryltriazole inhibitor (3b-190)
4XX7	1.77	Crystal structure of M2A mutant of human macrophage migration inhibitory factor
4XX8	1.77	Crystal structure of Pro1 deletion mutant of human macrophage migration inhibitory factor
4Z15	1.6	MIF in complex with 3-(2-furylmethyl)-2-thioxo-1,3-thiazolan-4-one
4Z1T	1.5	MIF in complex with 4-[(4-oxo-2-thioxo-1,3-thiazolan-3-yl)methyl]benzonitrile
4Z1U	2.05	MIF in complex with 1-(4-methylphenyl)-3-phenylprop-2-yn-1-one
5B4O	1.37	Crystal structure of Macrophage Migration Inhibitory Factor in complex with BTZO-14
5BS9	1.98	Crystal structure of N109A mutant of human macrophage migration inhibitory factor
5BSC	1.89	Crystal structure of K66A mutant of human macrophage migration inhibitory factor
5BSI	2.0	Crystal structure of Y36A mutant of human macrophage migration inhibitory factor
5BSJ	2.0	Crystal structure of S63A mutant of human macrophage migration inhibitory factor
5EIZ	1.96	Crystal structure of Y99A mutant of human macrophage migration inhibitory factor
5HVS	1.75	Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Biaryltriazole Inhibitor (3i-305)
5HVT	1.75	Crystal Structure of Macrophage Migration Inhibitory Factor (MIF) with a Potent Inhibitor (NVS-2)
5HVV	1.9	Crystal structure of Pro1 deletion and M2A double mutant of Macrophage Migration Inhibitory Factor
5J7P	1.85	Macrophage Migration Inhibitory Factor bound to Covalent Inhibitor RDR03785
5J7Q	2.05	Macrophage Migration Inhibitory Factor bound to Inhibitor K664 Derivative
5UMJ	1.61	Crystal structure of H62A mutant of human macrophage migration inhibitory factor
5UMK	1.73	Crystal structure of H62Y mutant of human macrophage migration inhibitory factor
5UZY	1.71	Crystal structure of N97A mutant of human macrophage migration inhibitory factor
5V70	1.94	Crystal structure of N102A mutant of human macrophage migration inhibitory factor
5V73	1.68	Crystal structure of N110A mutant of human macrophage migration inhibitory factor
5XEJ	2.5	Crystal Structure of Macrophage Migration Inhibitory Factor bound to MTX
6B1C	2.163	Macrophage Migration Inhibitory Factor in complex with a Naphthyridinone Inhibitor (4a)
6B1K	1.17	Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (3a)
6B2C	2.0	Macrophage Migration Inhibitory Factor in Complex with a Naphthyridinone Inhibitor (4b)
6BG6	1.52	Crystal structure of S111A mutant of human macrophage migration inhibitory factor
6BG7	2.54	Crystal structure of G107A mutant of human macrophage migration inhibitory factor
6CB5	1.78	Macrophage Migration Inhibitory Factor in complex with a Pyrazole Inhibitor (8g)
6CBF	2.3	Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (6a)
6CBG	2.0	Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (5)
6CBH	2.0	Macrophage Migration Inhibitory Factor in Complex with a Pyrazole Inhibitor (8m)
6FVE	1.41	Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound FITC
6FVH	1.4	Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound PITC
6OY8	1.53	Crystal structure of Y99G mutant of human macrophage migration inhibitory factor
6OYB	1.53	Crystal structure of H62G mutant of human macrophage migration inhibitory factor
6OYE	1.53	Crystal structure of Y99F mutant of human macrophage migration inhibitory factor
6OYG	1.55	Crystal structure of H62F mutant of human macrophage migration inhibitory factor
6PEG	2.0	MIF with a allosteric inhibitor
7E45	1.433	Crystal structure of compound 7 bound to MIF
7E47	1.38	Crystal structure of compound 6 bound to MIF
7E49	1.574	Crystal structure of MIF bound to compound10
7E4A	1.475	Crystal structure of MIF bound to compound 13
7E4B	1.772	Crystal structure of MIF bound to compound 5
7E4C	1.644	Crystal structure of MIF bound to compound11
7KQX	1.6	MIF Y99C homotrimeric mutant

GO term

GO ontology	GO term	GO description
Biological Process	GO:0030330	DNA damage response, signal transduction by p53 class mediator
Biological Process	GO:0019752	carboxylic acid metabolic process
Biological Process	GO:0007166	cell surface receptor signaling pathway
Biological Process	GO:0090398	cellular senescence
Biological Process	GO:0006954	inflammatory response
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0043518	negative regulation of DNA damage response, signal transduction by p53 class mediator
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0030336	negative regulation of cell migration
Biological Process	GO:2000773	negative regulation of cellular senescence
Biological Process	GO:0010629	negative regulation of gene expression
Biological Process	GO:1902166	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
Biological Process	GO:0010760	negative regulation of macrophage chemotaxis
Biological Process	GO:0002906	negative regulation of mature B cell apoptotic process
Biological Process	GO:0033033	negative regulation of myeloid cell apoptotic process
Biological Process	GO:0051248	negative regulation of protein metabolic process
Biological Process	GO:0030890	positive regulation of B cell proliferation
Biological Process	GO:0070374	positive regulation of ERK1 and ERK2 cascade
Biological Process	GO:0043406	positive regulation of MAP kinase activity
Biological Process	GO:0090238	positive regulation of arachidonic acid secretion
Biological Process	GO:0008284	positive regulation of cell population proliferation
Biological Process	GO:2000343	positive regulation of chemokine (C-X-C motif) ligand 2 production
Biological Process	GO:0001819	positive regulation of cytokine production
Biological Process	GO:0048146	positive regulation of fibroblast proliferation
Biological Process	GO:0031666	positive regulation of lipopolysaccharide-mediated signaling pathway
Biological Process	GO:0061081	positive regulation of myeloid leukocyte cytokine production involved in immune response
Biological Process	GO:0033138	positive regulation of peptidyl-serine phosphorylation
Biological Process	GO:0050731	positive regulation of peptidyl-tyrosine phosphorylation
Biological Process	GO:0042327	positive regulation of phosphorylation
Biological Process	GO:0061078	positive regulation of prostaglandin secretion involved in immune response
Biological Process	GO:0010739	positive regulation of protein kinase A signaling
Biological Process	GO:0032760	positive regulation of tumor necrosis factor production
Biological Process	GO:0001516	prostaglandin biosynthetic process
Biological Process	GO:0070207	protein homotrimerization
Biological Process	GO:0043030	regulation of macrophage activation
Molecular Function	GO:0042056	chemoattractant activity
Molecular Function	GO:0005125	cytokine activity
Molecular Function	GO:0005126	cytokine receptor binding
Molecular Function	GO:0004167	dopachrome isomerase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0050178	phenylpyruvate tautomerase activity
Molecular Function	GO:0002020	protease binding
Cellular Component	GO:0009986	cell surface
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005576	extracellular region
Cellular Component	GO:0005615	extracellular space
Cellular Component	GO:1904813	ficolin-1-rich granule lumen
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0034774	secretory granule lumen
Cellular Component	GO:0031982	vesicle

InterPro / Pfam

InterPro	InterPro name
IPR001398	Macrophage migration inhibitory factor
IPR014347	Tautomerase/MIF superfamily
IPR019829	Macrophage migration inhibitory factor, conserved site

Pfam	Pfam name
PF01187	Macrophage migration inhibitory factor (MIF)

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-202733	Cell surface interactions at the vascular wall	Internal node	R-HSA-109582	Hemostasis
R-HSA-6798695	Neutrophil degranulation	Leaf	R-HSA-168256	Immune System
R-HSA-8950505	Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation	Leaf	R-HSA-168256	Immune System

Subcellular location

Location	ECO term	Pubmed
Cytoplasm	ECO:0000269	PubMed:11089976
Cytoplasm	ECO:0000269	PubMed:19454686
Secreted	ECO:0000269	PubMed:15908412
Secreted	ECO:0000269	PubMed:19454686
Secreted	ECO:0000269	PubMed:2552447
Secreted	ECO:0000269	PubMed:8234256

Interactor | Variant | Drug

Phenotype

HPO ID	HPO name
HP:0000246	Sinusitis
HP:0000365	Hearing impairment
HP:0000716	Depression
HP:0000739	Anxiety
HP:0000787	Nephrolithiasis
HP:0000938	Osteopenia
HP:0000939	Osteoporosis
HP:0000988	Skin rash
HP:0001386	Joint swelling
HP:0001392	Abnormality of the liver
HP:0001394	Cirrhosis
HP:0001508	Failure to thrive
HP:0001701	Pericarditis
HP:0001738	Exocrine pancreatic insufficiency
HP:0001744	Splenomegaly
HP:0001945	Fever
HP:0002020	Gastroesophageal reflux
HP:0002024	Malabsorption
HP:0002027	Abdominal pain
HP:0002035	Rectal prolapse
HP:0002099	Asthma
HP:0002105	Hemoptysis
HP:0002107	Pneumothorax
HP:0002110	Bronchiectasis
HP:0002202	Pleural effusion
HP:0002205	Recurrent respiratory infections
HP:0002240	Hepatomegaly
HP:0002570	Steatorrhea
HP:0002716	Lymphadenopathy
HP:0002724	Recurrent Aspergillus infections
HP:0002726	Recurrent Staphylococcus aureus infections
HP:0002829	Arthralgia
HP:0002842	Recurrent Burkholderia cepacia infections
HP:0002910	Elevated hepatic transaminase
HP:0002960	Autoimmunity
HP:0003565	Elevated erythrocyte sedimentation rate
HP:0004401	Meconium ileus
HP:0005376	Recurrent Haemophilus influenzae infections
HP:0005681	Juvenile rheumatoid arthritis
HP:0006536	Airway obstruction
HP:0011227	Elevated circulating C-reactive protein concentration
HP:0012122	Anterior uveitis
HP:0012236	Elevated sweat chloride
HP:0012873	Absent vas deferens
HP:0032261	Nontuberculous mycobacterial pulmonary infection
HP:0032342	Reduced forced expiratory volume in one second
HP:0045082	Decreased body mass index
HP:0100582	Nasal polyposis

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adipose tissue	adipocytes	Medium	Approved
adrenal gland	glandular cells	Medium	Approved
appendix	lymphoid tissue	Low	Approved
bone marrow	hematopoietic cells	Low	Approved
breast	glandular cells	Medium	Approved
breast	myoepithelial cells	Medium	Approved
bronchus	respiratory epithelial cells	Medium	Approved
caudate	neuronal cells	Low	Approved
cerebellum	Purkinje cells	Low	Approved
cerebellum	cells in granular layer	Low	Approved
cerebral cortex	endothelial cells	Medium	Approved
cerebral cortex	glial cells	Low	Approved
cerebral cortex	neuronal cells	Medium	Approved
cerebral cortex	neuropil	Low	Approved
cervix	glandular cells	Medium	Approved
colon	endothelial cells	Low	Approved
colon	glandular cells	Medium	Approved
duodenum	glandular cells	Medium	Approved
endometrium 1	glandular cells	Medium	Approved
endometrium 2	glandular cells	Medium	Approved
epididymis	glandular cells	Medium	Approved
fallopian tube	glandular cells	Medium	Approved
gallbladder	glandular cells	Medium	Approved
hippocampus	neuronal cells	Medium	Approved
kidney	cells in glomeruli	Medium	Approved
kidney	cells in tubules	Medium	Approved
liver	cholangiocytes	Medium	Approved
liver	hepatocytes	Low	Approved
lung	macrophages	Medium	Approved
lymph node	germinal center cells	Low	Approved
nasopharynx	respiratory epithelial cells	Medium	Approved
oral mucosa	squamous epithelial cells	Low	Approved
ovary	ovarian stroma cells	Medium	Approved
pancreas	exocrine glandular cells	Medium	Approved
pancreas	pancreatic endocrine cells	Medium	Approved
parathyroid gland	glandular cells	Medium	Approved
placenta	trophoblastic cells	Medium	Approved
prostate	glandular cells	Medium	Approved
rectum	glandular cells	Low	Approved
salivary gland	glandular cells	Low	Approved
skin 1	keratinocytes	Low	Approved
skin 1	melanocytes	Low	Approved
skin 2	epidermal cells	Low	Approved
small intestine	glandular cells	Low	Approved
soft tissue 2	fibroblasts	Low	Approved
spleen	cells in red pulp	Low	Approved
spleen	cells in white pulp	Low	Approved
stomach 1	glandular cells	Medium	Approved
stomach 2	glandular cells	Medium	Approved
testis	Leydig cells	Medium	Approved
testis	cells in seminiferous ducts	Medium	Approved
thyroid gland	glandular cells	Medium	Approved
tonsil	germinal center cells	Medium	Approved
tonsil	non-germinal center cells	Low	Approved
urinary bladder	urothelial cells	Medium	Approved
vagina	squamous epithelial cells	Low	Approved

Summary of P14174

Gene: MIF, GLIF, MMIF
Protein: Macrophage migration inhibitory factor

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P14174

Gene: MIF, GLIF, MMIF Protein: Macrophage migration inhibitory factor

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: MIF, GLIF, MMIF
Protein: Macrophage migration inhibitory factor