MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.4.1.-	Transferases; Glycosyltransferases; Hexosyltransferases;
2.4.1.22	Transferases; Glycosyltransferases; Hexosyltransferases; lactose synthase
2.4.1.275	Transferases; Glycosyltransferases; Hexosyltransferases; neolactotriaosylceramide β-1,4-galactosyltransferase
2.4.1.38	Transferases; Glycosyltransferases; Hexosyltransferases; β-N-acetylglucosaminylglycopeptide β-1,4-galactosyltransferase
2.4.1.90	Transferases; Glycosyltransferases; Hexosyltransferases; N-acetyllactosamine synthase

Structure

PDB	Resolution (Å)	PDB name
2AE7	2.0	Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with Pentasaccharide
2AEC	2.0	Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,6-Man-beta-OR
2AES	2.0	Crystal Structure of Human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with GlcNAc-beta1,2-Man-alpha1,3-Man-beta-OR
2AGD	1.9	Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I(M340H-B4Gal-T1) in Complex with GlcNAc-beta1,4-Man-alpha1,3-Man-beta-OR
2AH9	2.0	Crystal Structure of Human M340H-Beta-1,4-Galactosyltransferase-I (M340H-B4Gal-T1) in Complex with Chitotriose
2FY7	1.7	Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in apo form
2FYA	1.9	Crystal structure of the catalytic domain of the human beta1, 4-galactosyltransferase mutant M339H complex with manganese
2FYB	1.9	Crystal structure of the catalytic domain of the human beta1,4-galactosyltransferase mutant M339H in complex with Mn and UDP-galactose in open conformation
3EE5	2.2	Crystal structure of human M340H-Beta1,4-Galactosyltransferase-I (M340H-B4GAL-T1) in complex with GLCNAC-Beta1,3-Gal-Beta-Naphthalenemethanol
4EE3	2.3	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with pentasaccharide
4EE4	1.95	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with tetrasaccharide from Lacto-N-neohexose
4EE5	2.2	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with trisaccharide from Lacto-N-neotetraose
4EEA	2.0	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta1,4-Glc-BETA
4EEG	2.2	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-Gal-Beta
4EEM	2.2	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-MAN-ALPHA-methyl
4EEO	2.3	Crystal structure of human M340H-beta-1,4-galactosyltransferase-1 (M340H-B4GAL-T1) in complex with GLCNAC-BETA1,6-GlcNAc-ALPHA-benzyl
4L41	2.7	Human Lactose synthase: A 2:1 complex between human alpha-lactalbumin and human beta1,4-galactosyltransferase
6FWT	1.845	Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-open monomeric form
6FWU	2.35	Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-closed dimeric form

GO term

GO ontology	GO term	GO description
Biological Process	GO:0002526	acute inflammatory response
Biological Process	GO:0060055	angiogenesis involved in wound healing
Biological Process	GO:0007339	binding of sperm to zona pellucida
Biological Process	GO:0007155	cell adhesion
Biological Process	GO:0045136	development of secondary sexual characteristics
Biological Process	GO:0002064	epithelial cell development
Biological Process	GO:0050673	epithelial cell proliferation
Biological Process	GO:0030198	extracellular matrix organization
Biological Process	GO:0006012	galactose metabolic process
Biological Process	GO:0005989	lactose biosynthetic process
Biological Process	GO:0006629	lipid metabolic process
Biological Process	GO:1905517	macrophage migration
Biological Process	GO:0050680	negative regulation of epithelial cell proliferation
Biological Process	GO:0009312	oligosaccharide biosynthetic process
Biological Process	GO:0007341	penetration of zona pellucida
Biological Process	GO:0043065	positive regulation of apoptotic process
Biological Process	GO:0060054	positive regulation of epithelial cell proliferation involved in wound healing
Biological Process	GO:0006487	protein N-linked glycosylation
Biological Process	GO:0060046	regulation of acrosome reaction
Molecular Function	GO:0003945	N-acetyllactosamine synthase activity
Molecular Function	GO:0035250	UDP-galactosyltransferase activity
Molecular Function	GO:0043014	alpha-tubulin binding
Molecular Function	GO:0003831	beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity
Molecular Function	GO:0048487	beta-tubulin binding
Molecular Function	GO:0008092	cytoskeletal protein binding
Molecular Function	GO:0008378	galactosyltransferase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0004461	lactose synthase activity
Molecular Function	GO:0030145	manganese ion binding
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0032580	Golgi cisterna membrane
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0000138	Golgi trans cisterna
Cellular Component	GO:0035577	azurophil granule membrane
Cellular Component	GO:0016323	basolateral plasma membrane
Cellular Component	GO:0031526	brush border membrane
Cellular Component	GO:0030057	desmosome
Cellular Component	GO:0009897	external side of plasma membrane
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005615	extracellular space
Cellular Component	GO:0030175	filopodium
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0030667	secretory granule membrane

InterPro / Pfam

InterPro	InterPro name
IPR003859	Beta-1,4-galactosyltransferase
IPR027791	Galactosyltransferase, C-terminal
IPR027995	Galactosyltransferase, N-terminal
IPR029044	Nucleotide-diphospho-sugar transferases

Pfam	Pfam name
PF02709	N-terminal domain of galactosyltransferase
PF13733	N-terminal region of glycosyl transferase group 7

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1912420	Pre-NOTCH Processing in Golgi	Leaf	R-HSA-162582	Signal Transduction
R-HSA-2022854	Keratan sulfate biosynthesis	Leaf	R-HSA-1430728	Metabolism
R-HSA-2534343	Interaction With Cumulus Cells And The Zona Pellucida	Leaf	R-HSA-1474165	Reproduction
R-HSA-3656244	Defective B4GALT1 causes B4GALT1-CDG (CDG-2d)	Leaf	R-HSA-1643685	Disease
R-HSA-4793953	Defective B4GALT1 causes CDG-2d	Leaf	R-HSA-1643685	Disease
R-HSA-5653890	Lactose synthesis	Leaf	R-HSA-1430728	Metabolism
R-HSA-6798695	Neutrophil degranulation	Leaf	R-HSA-168256	Immune System
R-HSA-975577	N-Glycan antennae elongation	Leaf	R-HSA-392499	Metabolism of proteins

Subcellular location

Location	ECO term	Pubmed
Cell membrane	ECO:0000269	PubMed:1714903
Cell projection, filopodium	ECO:0000250
Cell surface	ECO:0000269	PubMed:1714903
Golgi apparatus, Golgi stack membrane	ECO:0000269	PubMed:1714903
Golgi apparatus, Golgi stack membrane	ECO:0000269	PubMed:20378551
Secreted	ECO:0000303	PubMed:2120039

Interactor | Variant | Drug

Phenotype

HPO ID	HPO name
HP:0000007	Autosomal recessive inheritance
HP:0000219	Thin upper lip vermilion
HP:0000238	Hydrocephalus
HP:0000256	Macrocephaly
HP:0000316	Hypertelorism
HP:0000343	Long philtrum
HP:0000369	Low-set ears
HP:0000431	Wide nasal bridge
HP:0000545	Myopia
HP:0000821	Hypothyroidism
HP:0000969	Edema
HP:0001252	Hypotonia
HP:0001263	Global developmental delay
HP:0001305	Dandy-Walker malformation
HP:0001321	Cerebellar hypoplasia
HP:0001518	Small for gestational age
HP:0001622	Premature birth
HP:0001744	Splenomegaly
HP:0001892	Abnormal bleeding
HP:0001976	Reduced antithrombin III activity
HP:0001999	Abnormal facial shape
HP:0002014	Diarrhea
HP:0002240	Hepatomegaly
HP:0002910	Elevated hepatic transaminase
HP:0003160	Abnormal isoelectric focusing of serum transferrin
HP:0003186	Inverted nipples
HP:0003198	Myopathy
HP:0003199	Decreased muscle mass
HP:0003236	Elevated circulating creatine kinase concentration
HP:0003256	Abnormality of the coagulation cascade
HP:0003563	Decreased LDL cholesterol concentration
HP:0003577	Congenital onset
HP:0003645	Prolonged partial thromboplastin time
HP:0004855	Reduced protein S activity
HP:0005543	Reduced protein C activity
HP:0005989	Redundant neck skin
HP:0008947	Infantile muscular hypotonia
HP:0009062	Infantile axial hypotonia
HP:0011003	High myopia
HP:0011123	Inflammatory abnormality of the skin
HP:0012301	Type II transferrin isoform profile
HP:0012379	Abnormal circulating enzyme concentration or activity
HP:0031956	Elevated circulating aspartate aminotransferase concentration

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	High	Enhanced
appendix	glandular cells	High	Enhanced
appendix	lymphoid tissue	Medium	Enhanced
bone marrow	hematopoietic cells	Medium	Enhanced
breast	glandular cells	Medium	Enhanced
bronchus	basal cells	High	Enhanced
bronchus	ciliated cells (cell body)	High	Enhanced
bronchus	goblet cells	High	Enhanced
cerebral cortex	neuronal cells	Low	Enhanced
cervix	glandular cells	High	Enhanced
cervix	squamous epithelial cells	Low	Enhanced
colon	glandular cells	High	Enhanced
duodenum	glandular cells	High	Enhanced
endometrium 1	cells in endometrial stroma	Low	Enhanced
endometrium 1	glandular cells	High	Enhanced
endometrium 2	glandular cells	High	Enhanced
epididymis	glandular cells	High	Enhanced
esophagus	squamous epithelial cells	Medium	Enhanced
fallopian tube	ciliated cells (cell body)	High	Enhanced
fallopian tube	non-ciliated cells	High	Enhanced
gallbladder	glandular cells	High	Enhanced
kidney	cells in glomeruli	Medium	Enhanced
kidney	cells in tubules	High	Enhanced
liver	cholangiocytes	Medium	Enhanced
liver	hepatocytes	High	Enhanced
lung	alveolar cells type I	Medium	Enhanced
lung	macrophages	Low	Enhanced
lymph node	germinal center cells	High	Enhanced
lymph node	non-germinal center cells	High	Enhanced
nasopharynx	basal cells	High	Enhanced
nasopharynx	ciliated cells (cell body)	High	Enhanced
nasopharynx	goblet cells	High	Enhanced
oral mucosa	squamous epithelial cells	Medium	Enhanced
pancreas	exocrine glandular cells	Medium	Enhanced
pancreas	pancreatic endocrine cells	Low	Enhanced
parathyroid gland	glandular cells	High	Enhanced
placenta	decidual cells	High	Enhanced
placenta	trophoblastic cells	High	Enhanced
prostate	glandular cells	High	Enhanced
rectum	glandular cells	High	Enhanced
salivary gland	glandular cells	Medium	Enhanced
seminal vesicle	glandular cells	High	Enhanced
skin 1	eccrine glands	Medium	Enhanced
skin 1	endothelial cells	Medium	Enhanced
skin 1	fibrohistiocytic cells	High	Enhanced
skin 1	lymphocytes	Low	Enhanced
skin 1	melanocytes	Medium	Enhanced
skin 2	cells in basal layer	Medium	Enhanced
skin 2	endothelial cells	Medium	Enhanced
skin 2	fibrohistiocytic cells	High	Enhanced
skin 2	lymphocytes	Low	Enhanced
skin 2	melanocytes	High	Enhanced
small intestine	glandular cells	High	Enhanced
spleen	cells in red pulp	Medium	Enhanced
spleen	cells in white pulp	Medium	Enhanced
stomach 1	glandular cells	High	Enhanced
stomach 2	glandular cells	High	Enhanced
testis	Leydig cells	High	Enhanced
testis	sertoli cells	Medium	Enhanced
thyroid gland	glandular cells	Medium	Enhanced
tonsil	germinal center cells	High	Enhanced
tonsil	non-germinal center cells	Medium	Enhanced
tonsil	squamous epithelial cells	High	Enhanced
urinary bladder	urothelial cells	Medium	Enhanced
vagina	squamous epithelial cells	Low	Enhanced

Summary of P15291

Gene: B4GALT1, GGTB2
Protein: Beta-1,4-galactosyltransferase 1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P15291

Gene: B4GALT1, GGTB2 Protein: Beta-1,4-galactosyltransferase 1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: B4GALT1, GGTB2
Protein: Beta-1,4-galactosyltransferase 1