MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.7.10.2	Transferases; Transferring phosphorus-containing groups; Protein-tyrosine kinases; non-specific protein-tyrosine kinase
2.7.11.1	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; non-specific serine/threonine protein kinase

Structure

PDB	Resolution (Å)	PDB name
1QU6		STRUCTURE OF THE DOUBLE-STRANDED RNA-BINDING DOMAIN OF THE PROTEIN KINASE PKR REVEALS THE MOLECULAR BASIS OF ITS DSRNA-MEDIATED ACTIVATION
2A19	2.5	PKR kinase domain- eIF2alpha- AMP-PNP complex.
2A1A	2.8	PKR kinase domain-eIF2alpha Complex
3UIU	2.903	Crystal structure of Apo-PKR kinase domain
6D3K	2.6	Crystal structure of unphosphorylated human PKR kinase domain in complex with ADP
6D3L	3.1	Crystal structure of unphosphorylated human PKR
7OBK	1.8	Crystal structure of 14-3-3 sigma in complex with PKR phosphopeptide
7OBL	1.8	Crystal structure of 14-3-3 sigma in complex with PKR phosphopeptide and stabilizer Fusicoccin-A
8BI7	1.4	Binary structure of 14-3-3s and PKR phosphopeptide

GO term

GO ontology	GO term	GO description
Biological Process	GO:0140374	antiviral innate immune response
Biological Process	GO:0034198	cellular response to amino acid starvation
Biological Process	GO:0051607	defense response to virus
Biological Process	GO:0030968	endoplasmic reticulum unfolded protein response
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0008285	negative regulation of cell population proliferation
Biological Process	GO:0033689	negative regulation of osteoblast proliferation
Biological Process	GO:0017148	negative regulation of translation
Biological Process	GO:0045071	negative regulation of viral genome replication
Biological Process	GO:0043410	positive regulation of MAPK cascade
Biological Process	GO:0051092	positive regulation of NF-kappaB transcription factor activity
Biological Process	GO:1901224	positive regulation of NIK/NF-kappaB signaling
Biological Process	GO:0032722	positive regulation of chemokine production
Biological Process	GO:0001819	positive regulation of cytokine production
Biological Process	GO:0032874	positive regulation of stress-activated MAPK cascade
Biological Process	GO:0046777	protein autophosphorylation
Biological Process	GO:0006468	protein phosphorylation
Biological Process	GO:1900225	regulation of NLRP3 inflammasome complex assembly
Biological Process	GO:1901532	regulation of hematopoietic progenitor cell differentiation
Biological Process	GO:1902036	regulation of hematopoietic stem cell differentiation
Biological Process	GO:1902033	regulation of hematopoietic stem cell proliferation
Biological Process	GO:0035455	response to interferon-alpha
Biological Process	GO:0009615	response to virus
Biological Process	GO:0006412	translation
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003725	double-stranded RNA binding
Molecular Function	GO:0004694	eukaryotic translation initiation factor 2alpha kinase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0016301	kinase activity
Molecular Function	GO:0140677	molecular function activator activity
Molecular Function	GO:0004715	non-membrane spanning protein tyrosine kinase activity
Molecular Function	GO:0004672	protein kinase activity
Molecular Function	GO:0019888	protein phosphatase regulator activity
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0005840	ribosome

InterPro / Pfam

InterPro	InterPro name
IPR000719	Protein kinase domain
IPR008271	Serine/threonine-protein kinase, active site
IPR011009	Protein kinase-like domain superfamily
IPR014720	Double-stranded RNA-binding domain
IPR017441	Protein kinase, ATP binding site
IPR044452	EIF2AK2, first double-stranded RNA binding domain
IPR044453	EIF2AK2, second double-stranded RNA binding domain

Pfam	Pfam name
PF00035	Double-stranded RNA binding motif
PF00069	Protein kinase domain

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1169408	ISG15 antiviral mechanism	Leaf	R-HSA-168256	Immune System
R-HSA-169131	Inhibition of PKR	Leaf	R-HSA-1643685	Disease

Subcellular location

Location	ECO term	Pubmed
Cytoplasm	ECO:0000269	PubMed:15121867
Cytoplasm	ECO:0000269	PubMed:21029237
Cytoplasm	ECO:0000269	PubMed:22214662
Cytoplasm, perinuclear region	ECO:0000269	PubMed:15121867
Nucleus	ECO:0000269	PubMed:21029237
Nucleus	ECO:0000269	PubMed:21072047

Interactor | Variant | Drug

Phenotype

HPO ID	HPO name
HP:0000006	Autosomal dominant inheritance
HP:0000020	Urinary incontinence
HP:0000252	Microcephaly
HP:0000298	Mask-like facies
HP:0000511	Vertical supranuclear gaze palsy
HP:0001249	Intellectual disability
HP:0001250	Seizure
HP:0001252	Hypotonia
HP:0001257	Spasticity
HP:0001260	Dysarthria
HP:0001263	Global developmental delay
HP:0001266	Choreoathetosis
HP:0001276	Hypertonia
HP:0001288	Gait disturbance
HP:0001300	Parkinsonism
HP:0001332	Dystonia
HP:0001336	Myoclonus
HP:0001337	Tremor
HP:0001344	Absent speech
HP:0001608	Abnormality of the voice
HP:0001761	Pes cavus
HP:0002059	Cerebral atrophy
HP:0002063	Rigidity
HP:0002066	Gait ataxia
HP:0002067	Bradykinesia
HP:0002078	Truncal ataxia
HP:0002079	Hypoplasia of the corpus callosum
HP:0002126	Polymicrogyria
HP:0002188	Delayed CNS myelination
HP:0002451	Limb dystonia
HP:0002530	Axial dystonia
HP:0002540	Inability to walk
HP:0002578	Gastroparesis
HP:0002599	Head titubation
HP:0002607	Bowel incontinence
HP:0003011	Abnormality of the musculature
HP:0003487	Babinski sign
HP:0003593	Infantile onset
HP:0003621	Juvenile onset
HP:0008936	Axial hypotonia
HP:0011463	Childhood onset
HP:0025708	Early young adult onset
HP:0032807	Neonatal seizure
HP:0100022	Abnormality of movement
HP:0100248	Hemiballismus

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	High	Approved
appendix	glandular cells	Low	Approved
appendix	lymphoid tissue	Low	Approved
bone marrow	hematopoietic cells	High	Approved
breast	glandular cells	High	Approved
breast	myoepithelial cells	High	Approved
bronchus	respiratory epithelial cells	High	Approved
caudate	glial cells	Low	Approved
caudate	neuronal cells	High	Approved
cerebellum	Purkinje cells	High	Approved
cerebral cortex	endothelial cells	Medium	Approved
cerebral cortex	glial cells	Medium	Approved
cerebral cortex	neuronal cells	High	Approved
cerebral cortex	neuropil	Low	Approved
cervix	glandular cells	High	Approved
cervix	squamous epithelial cells	Medium	Approved
colon	endothelial cells	Medium	Approved
colon	glandular cells	High	Approved
duodenum	glandular cells	High	Approved
endometrium 1	cells in endometrial stroma	High	Approved
endometrium 1	glandular cells	High	Approved
endometrium 2	cells in endometrial stroma	Medium	Approved
endometrium 2	glandular cells	High	Approved
epididymis	glandular cells	High	Approved
esophagus	squamous epithelial cells	Medium	Approved
fallopian tube	glandular cells	High	Approved
gallbladder	glandular cells	High	Approved
heart muscle	cardiomyocytes	Low	Approved
hippocampus	glial cells	Low	Approved
hippocampus	neuronal cells	High	Approved
kidney	cells in glomeruli	Low	Approved
kidney	cells in tubules	Medium	Approved
liver	cholangiocytes	Low	Approved
liver	hepatocytes	Medium	Approved
lung	alveolar cells	Low	Approved
lung	macrophages	Low	Approved
lymph node	germinal center cells	Low	Approved
lymph node	non-germinal center cells	Medium	Approved
nasopharynx	respiratory epithelial cells	High	Approved
oral mucosa	squamous epithelial cells	Medium	Approved
ovary	follicle cells	Low	Approved
ovary	ovarian stroma cells	Low	Approved
pancreas	exocrine glandular cells	High	Approved
pancreas	pancreatic endocrine cells	Low	Approved
parathyroid gland	glandular cells	High	Approved
placenta	decidual cells	High	Approved
placenta	trophoblastic cells	Medium	Approved
prostate	glandular cells	Medium	Approved
rectum	glandular cells	High	Approved
salivary gland	glandular cells	High	Approved
seminal vesicle	glandular cells	High	Approved
skeletal muscle	myocytes	Low	Approved
skin 1	fibroblasts	Low	Approved
skin 1	keratinocytes	Medium	Approved
skin 1	melanocytes	Low	Approved
skin 2	epidermal cells	High	Approved
small intestine	glandular cells	High	Approved
smooth muscle	smooth muscle cells	Low	Approved
soft tissue 1	fibroblasts	Medium	Approved
soft tissue 2	fibroblasts	Medium	Approved
soft tissue 2	peripheral nerve	Low	Approved
spleen	cells in red pulp	Low	Approved
stomach 1	glandular cells	Medium	Approved
testis	Leydig cells	High	Approved
testis	cells in seminiferous ducts	Medium	Approved
thyroid gland	glandular cells	High	Approved
tonsil	germinal center cells	High	Approved
tonsil	non-germinal center cells	Medium	Approved
tonsil	squamous epithelial cells	Low	Approved
urinary bladder	urothelial cells	High	Approved
vagina	squamous epithelial cells	Medium	Approved

Disease

Summary of P19525

Gene: EIF2AK2, PKR, PRKR
Protein: Interferon-induced, double-stranded RNA-activated protein kinase

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P19525

Gene: EIF2AK2, PKR, PRKR Protein: Interferon-induced, double-stranded RNA-activated protein kinase

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: EIF2AK2, PKR, PRKR
Protein: Interferon-induced, double-stranded RNA-activated protein kinase