MultifacetedProtDB - Bologna Biocomputing Group

Catalytic activity

EC number	EC number description
1.14.14.23	Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor; cholesterol 7α-monooxygenase
1.14.14.26	Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor; 24-hydroxycholesterol 7α-hydroxylase

Publications

PDB	Resolution (Å)	PDB name
3DAX	2.15	Crystal structure of human CYP7A1
3SN5	2.75	Crystal structure of human CYP7A1 in complex with cholest-4-en-3-one
3V8D	1.9	Crystal structure of human CYP7A1 in complex with 7-ketocholesterol

GO ontology	GO term	GO description
Biological Process	GO:0015721	bile acid and bile salt transport
Biological Process	GO:0006699	bile acid biosynthetic process
Biological Process	GO:0038183	bile acid signaling pathway
Biological Process	GO:0071397	cellular response to cholesterol
Biological Process	GO:0071333	cellular response to glucose stimulus
Biological Process	GO:0006707	cholesterol catabolic process
Biological Process	GO:0042632	cholesterol homeostasis
Biological Process	GO:0032966	negative regulation of collagen biosynthetic process
Biological Process	GO:0045717	negative regulation of fatty acid biosynthetic process
Biological Process	GO:0045542	positive regulation of cholesterol biosynthetic process
Biological Process	GO:0070857	regulation of bile acid biosynthetic process
Biological Process	GO:0010468	regulation of gene expression
Biological Process	GO:0045471	response to ethanol
Biological Process	GO:0016125	sterol metabolic process
Molecular Function	GO:0033782	24-hydroxycholesterol 7alpha-hydroxylase activity
Molecular Function	GO:0008123	cholesterol 7-alpha-monooxygenase activity
Molecular Function	GO:0020037	heme binding
Molecular Function	GO:0005506	iron ion binding
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0043231	intracellular membrane-bounded organelle

InterPro	InterPro name
IPR001128	Cytochrome P450
IPR002403	Cytochrome P450, E-class, group IV
IPR017972	Cytochrome P450, conserved site
IPR024204	Cytochrome P450, cholesterol 7-alpha-monooxygenase-type
IPR030681	Cholesterol 7-alpha-monooxygenase
IPR036396	Cytochrome P450 superfamily

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-192105	Synthesis of bile acids and bile salts	Internal node	R-HSA-1430728	Metabolism
R-HSA-193368	Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol	Leaf	R-HSA-1430728	Metabolism
R-HSA-193807	Synthesis of bile acids and bile salts via 27-hydroxycholesterol	Leaf	R-HSA-1430728	Metabolism
R-HSA-1989781	PPARA activates gene expression	Leaf	R-HSA-1430728	Metabolism
R-HSA-211976	Endogenous sterols	Internal node	R-HSA-1430728	Metabolism

Location	ECO term	Pubmed
Endoplasmic reticulum membrane	ECO:0000305	PubMed:11013305
Endoplasmic reticulum membrane	ECO:0000305	PubMed:2384150
Microsome membrane	ECO:0000305	PubMed:11013305
Microsome membrane	ECO:0000305	PubMed:2384150

HPO ID	HPO name
HP:0001396	Cholestasis
HP:0001397	Hepatic steatosis
HP:0001403	Macrovesicular hepatic steatosis
HP:0001513	Obesity
HP:0001677	Coronary artery atherosclerosis
HP:0002155	Hypertriglyceridemia
HP:0003124	Hypercholesterolemia
HP:0003141	Increased LDL cholesterol concentration
HP:0004943	Accelerated atherosclerosis
HP:0006573	Acute hepatic steatosis
HP:0008372	Abnormality of vitamin A metabolism
HP:0011980	Cholesterol gallstones
HP:0012115	Hepatitis
HP:0012397	Aortic atherosclerotic lesion
HP:0100514	Abnormality of vitamin E metabolism