MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
4.4.1.1	Lyases; Carbon-sulfur lyases; Carbon-sulfur lyases (only sub-subclass identified to date); cystathionine γ-lyase
4.4.1.2	Lyases; Carbon-sulfur lyases; Carbon-sulfur lyases (only sub-subclass identified to date); homocysteine desulfhydrase

Structure

PDB	Resolution (Å)	PDB name
2NMP	2.6	Crystal structure of human Cystathionine gamma lyase
3COG	2.0	Crystal structure of human cystathionase (Cystathionine gamma lyase) in complex with DL-propargylglycine
3ELP	2.4	Structure of cystationine gamma lyase
5EIG	2.7	Engineered human cystathionine gamma lyase (E59T, E339V) to deplet cysteine
5TSU	2.2	Active conformation for Engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine
5TT2	2.949	Inactive conformation of engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine
6NBA	2.495	Crystal structure of Human Cystathionine gamma lyase with S-3-Carboxpropyl-L-Cysteine
6OVG	2.719	L-Methionine Depletion with an Engineered Human Enzyme Disrupts Prostate Cancer Metabolism

GO term

GO ontology	GO term	GO description
Biological Process	GO:1990830	cellular response to leukemia inhibitory factor
Biological Process	GO:0019344	cysteine biosynthetic process
Biological Process	GO:0019343	cysteine biosynthetic process via cystathionine
Biological Process	GO:0006534	cysteine metabolic process
Biological Process	GO:0030968	endoplasmic reticulum unfolded protein response
Biological Process	GO:0070814	hydrogen sulfide biosynthetic process
Biological Process	GO:0006629	lipid metabolic process
Biological Process	GO:2001234	negative regulation of apoptotic signaling pathway
Biological Process	GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
Biological Process	GO:0051092	positive regulation of NF-kappaB transcription factor activity
Biological Process	GO:1904831	positive regulation of aortic smooth muscle cell differentiation
Biological Process	GO:0051289	protein homotetramerization
Biological Process	GO:0044524	protein sulfhydration
Biological Process	GO:0018272	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
Biological Process	GO:0019346	transsulfuration
Molecular Function	GO:0080146	L-cysteine desulfhydrase activity
Molecular Function	GO:0044540	L-cystine L-cysteine-lyase (deaminating)
Molecular Function	GO:0005516	calmodulin binding
Molecular Function	GO:0004123	cystathionine gamma-lyase activity
Molecular Function	GO:0047982	homocysteine desulfhydrase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0030170	pyridoxal phosphate binding
Molecular Function	GO:0098606	selenocystathionine gamma-lyase activity
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0070062	extracellular exosome

InterPro / Pfam

InterPro	InterPro name
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme
IPR015421	Pyridoxal phosphate-dependent transferase, major domain
IPR015422	Pyridoxal phosphate-dependent transferase, small domain
IPR015424	Pyridoxal phosphate-dependent transferase

Pfam	Pfam name
PF01053	Cys/Met metabolism PLP-dependent enzyme

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1614558	Degradation of cysteine and homocysteine	Internal node	R-HSA-1430728	Metabolism
R-HSA-1614603	Cysteine formation from homocysteine	Leaf	R-HSA-1430728	Metabolism
R-HSA-2408508	Metabolism of ingested SeMet, Sec, MeSec into H2Se	Leaf	R-HSA-1430728	Metabolism

Subcellular location

Location	ECO term	Pubmed
Cytoplasm

Interactor | Variant | Drug

Phenotype

HPO ID	HPO name
HP:0000007	Autosomal recessive inheritance
HP:0000787	Nephrolithiasis
HP:0001249	Intellectual disability
HP:0001250	Seizure
HP:0001337	Tremor
HP:0001762	Talipes equinovarus
HP:0003153	Cystathioninuria
HP:0003286	Cystathioninemia
HP:0008572	External ear malformation

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Low	Enhanced
cerebellum	Bergmann glia - cytoplasm/membrane	High	Enhanced
cerebellum	molecular layer cells - cytoplasm/membrane	Low	Enhanced
cerebral cortex	neuronal cells	Low	Enhanced
hippocampus	neuronal cells	Low	Enhanced
kidney	cells in tubules	Low	Enhanced
liver	hepatocytes	High	Enhanced
lung	alveolar cells type II	Low	Enhanced
lung	macrophages	Low	Enhanced
pancreas	exocrine glandular cells	Low	Enhanced
skin 1	melanocytes	Low	Enhanced
skin 2	epidermal cells	Low	Enhanced
stomach 1	glandular cells	Low	Enhanced
stomach 2	glandular cells	Low	Enhanced
testis	Leydig cells	Low	Enhanced
testis	sertoli cells	Medium	Enhanced

Summary of P32929

Gene: CTH
Protein: Cystathionine gamma-lyase

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P32929

Gene: CTH Protein: Cystathionine gamma-lyase

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: CTH
Protein: Cystathionine gamma-lyase