MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.7.11.21	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; polo kinase

Structure

PDB	Resolution (Å)	PDB name
1Q4K	2.3	The polo-box domain of Plk1 in complex with a phospho-peptide
1Q4O	2.2	The structure of the polo box domain of human Plk1
1UMW	1.9	Structure of a human Plk1 Polo-box domain/phosphopeptide complex
2OGQ	1.95	Molecular and structural basis of Plk1 substrate recognition: Implications in centrosomal localization
2OJX	2.85	Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization
2OU7	2.4	Structure of the Catalytic Domain of Human Polo-like Kinase 1
2OWB	2.1	Structure of the Catalytic Domain of Human Polo-like Kinase 1
2RKU	1.95	Structure of PLK1 in complex with BI2536
2V5Q	2.3	CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN
2YAC	2.2	Crystal structure of Polo-like kinase 1 in complex with NMS-P937
3BZI	2.1	Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization
3C5L	2.33	Polo-like kinase 1 Polo box domain in complex with PPHSpT peptide
3FC2	2.45	PLK1 in complex with BI6727
3FVH	1.58	Polo-like kinase 1 Polo box domain in complex with Ac-LHSpTA-NH2 peptide
3HIH	1.7	Structure of human Plk1-PBD with glycerol and sulfate in the phophopeptide binding site
3HIK	1.77	Structure of human Plk1-PBD in complex with PLHSpT
3KB7	2.5	Crystal structure of Polo-like kinase 1 in complex with a pyrazoloquinazoline inhibitor
3P2W	1.66	Unliganded form of Polo-like kinase I Polo-box domain
3P2Z	1.79	Polo-like kinase I Polo-box domain in complex with PLHSpTA phosphopeptide from PBIP1
3P34	1.4	Polo-like kinase I Polo-box domain in complex with MQSpTPL phosphopeptide
3P35	2.09	Polo-like kinase I Polo-box domain in complex with MQSpSPL phosphopeptide
3P36	1.59	Polo-like kinase I Polo-box domain in complex with DPPLHSpTA phosphopeptide from PBIP1
3P37	2.38	Polo-like kinase I Polo-box domain in complex with FDPPLHSpTA phosphopeptide from PBIP1
3Q1I	1.4	Polo-like kinase I Polo-box domain in complex with FMPPPMSpSM phosphopeptide from TCERG1
3RQ7	1.55	Polo-like kinase 1 Polo box domain in complex with a C6H5(CH2)8-derivatized peptide inhibitor
3THB	2.5	Structure of PLK1 kinase domain in complex with a benzolactam-derived inhibitor
4A4L	2.35	CRYSTAL STRUCTURE OF POLO-LIKE KINASE 1 IN COMPLEX WITH A 5-(2-AMINO- PYRIMIDIN-4-YL)-1H-PYRROLE INHIBITOR
4A4O	2.7	CRYSTAL STRUCTURE OF POLO-LIKE KINASE 1 IN COMPLEX WITH A 2-(2-AMINO- PYRIMIDIN-4-YL)-1,5,6,7-TETRAHYDRO-PYRROLOPYRIDIN-4-ONE INHIBITOR
4DFW	1.55	Oxime-based Post Solid-phase Peptide Diversification: Identification of High Affinity Polo-like Kinase 1 (Plk1) Polo-box Domain Binding Peptides
4E67	2.1	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with hydrocinnamoyl-derivatized PLHSpTA peptide
4E9C	1.7	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LDPPLHSpTA phosphopeptide
4E9D	2.75	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with 3-(1-benzothiophen-2-yl)propanoyl-derivatized DPPLHSpTA peptide
4H5X	1.95	human Plk1-PBD with a glycerol bound at the phophopeptide binding site
4H71	1.93	Human Plk1-PBD in complex with Poloxime ((E)-4-(hydroxyimino)-2-isopropyl-5-methylcyclohexa-2,5-dienone)
4HAB	2.65	Crystal structure of Plk1 Polo-box domain in complex with PL-49
4HCO	2.75	Human Plk1-PBD in complex with Thymoquinone at the phophopeptide binding site
4HY2	2.0	Crystal structure of Plk1 Polo-box domain in complex with PL-42
4J52	2.3	Crystal structure of PLK1 in complex with a pyrimidodiazepinone inhibitor
4J53	2.5	Crystal structure of PLK1 in complex with TAK-960
4LKL	1.58	Crystal structure of Plk1 Polo-box domain in complex with PL-55
4LKM	2.0	Crystal structure of Plk1 Polo-box domain in complex with PL-74
4O56	1.8	Structure of PLK1 in complex with peptide
4O6W	1.448	Peptide-Based Inhibitors of Plk1 Polo-box Domain
4O9W	1.694	Crystal structure of polo-like kinase(PLK1)PBD in complex with phospho peptide
4RCP	1.6	Crystal structure of Plk1 Polo-box domain in complex with PL-2
4WHH	1.9	A New Class of Peptidomimetics Targeting the Polo-box Domain of Polo-like kinase 1
4WHK	1.8	A New Class of Peptidomimetics Targeting the Polo-box Domain of Polo-like kinase 1
4WHL	2.71	A New Class of Peptidomimetics Targeting the Polo-box Domain of Polo-like kinase 1
4X9R	1.398	PLK-1 polo-box domain in complex with Bioactive Imidazolium-containing phosphopeptide macrocycle 3B
4X9V	1.429	PLK-1 polo-box domain in complex with Bioactive Imidazolium-containing phosphopeptide macrocycle 3C
4X9W	1.798	PLK-1 polo-box domain in complex with Bioactive Imidazolium-containing phosphopeptide macrocycle 4C
5J19	2.0	phospho-Pon binding-induced Plk1 dimerization
5NEI	2.68	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with JES107
5NFU	1.809	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LHSpTA peptide
5NJE	1.978	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with Alpha-Bromo-3-Iodotoluene.
5NMM	2.02	The structure of the polo-box domain (PBD) of Plk1 in complex with Alpha-Bromo-3-Iodotoluene.
5NN1	1.785	The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1)
5NN2	1.81	The structure of the polo-box domain (PBD) of Plk1 in complex with Z228588490
5TA6	2.5	Crystal structure of PLK1 in complex with a novel 5,6-dihydroimidazolo[1,5-f]pteridine inhibitor.
5TA8	2.6	Crystal structure of PLK1 in complex with a novel 5,6-dihydroimidazolo[1,5-f]pteridine inhibitor
6AX4	1.45	Plk-1 polo-box domain in complex with histidine N(tau)-cyclized Macrocycle 5b.
6GY2	3.11	Crystal structure of human Plk1-PBD in complex with WSSSLATPPTLSSpTVLI phosphopeptide from BRCA2
7MSO	1.85	Crystal Structure of Polo Box Domain in Complex with Cyclic Peptide Inhibitor
7MX1	1.64	PLK-1 polo-box domain in complex with a high affinity macrocycle synthesized using a novel glutamic acid analog

GO term

GO ontology	GO term	GO description
Biological Process	GO:0000086	G2/M transition of mitotic cell cycle
Biological Process	GO:0007098	centrosome cycle
Biological Process	GO:0000132	establishment of mitotic spindle orientation
Biological Process	GO:0045184	establishment of protein localization
Biological Process	GO:0016321	female meiosis chromosome segregation
Biological Process	GO:0045143	homologous chromosome segregation
Biological Process	GO:0001578	microtubule bundle formation
Biological Process	GO:0007095	mitotic G2 DNA damage checkpoint signaling
Biological Process	GO:0000278	mitotic cell cycle
Biological Process	GO:0007076	mitotic chromosome condensation
Biological Process	GO:0000281	mitotic cytokinesis
Biological Process	GO:0007077	mitotic nuclear membrane disassembly
Biological Process	GO:0000070	mitotic sister chromatid segregation
Biological Process	GO:0007094	mitotic spindle assembly checkpoint signaling
Biological Process	GO:0007052	mitotic spindle organization
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0045736	negative regulation of cyclin-dependent protein serine/threonine kinase activity
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:0051081	nuclear membrane disassembly
Biological Process	GO:0018105	peptidyl-serine phosphorylation
Biological Process	GO:0010800	positive regulation of peptidyl-threonine phosphorylation
Biological Process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process	GO:1900182	positive regulation of protein localization to nucleus
Biological Process	GO:0045862	positive regulation of proteolysis
Biological Process	GO:1904668	positive regulation of ubiquitin protein ligase activity
Biological Process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
Biological Process	GO:0031648	protein destabilization
Biological Process	GO:0071168	protein localization to chromatin
Biological Process	GO:0090435	protein localization to nuclear envelope
Biological Process	GO:0006468	protein phosphorylation
Biological Process	GO:0016567	protein ubiquitination
Biological Process	GO:1905784	regulation of anaphase-promoting complex-dependent catabolic process
Biological Process	GO:0051726	regulation of cell cycle
Biological Process	GO:0032465	regulation of cytokinesis
Biological Process	GO:0007346	regulation of mitotic cell cycle
Biological Process	GO:1901990	regulation of mitotic cell cycle phase transition
Biological Process	GO:0030071	regulation of mitotic metaphase/anaphase transition
Biological Process	GO:1901673	regulation of mitotic spindle assembly
Biological Process	GO:0043393	regulation of protein binding
Biological Process	GO:1904776	regulation of protein localization to cell cortex
Biological Process	GO:0007062	sister chromatid cohesion
Biological Process	GO:0070194	synaptonemal complex disassembly
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0010997	anaphase-promoting complex binding
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0000287	magnesium ion binding
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0004672	protein kinase activity
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Cellular Component	GO:0034451	centriolar satellite
Cellular Component	GO:0005814	centriole
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0000785	chromatin
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0000776	kinetochore
Cellular Component	GO:0015630	microtubule cytoskeleton
Cellular Component	GO:0030496	midbody
Cellular Component	GO:0097431	mitotic spindle pole
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0000940	outer kinetochore
Cellular Component	GO:0005819	spindle
Cellular Component	GO:0051233	spindle midzone
Cellular Component	GO:0000922	spindle pole
Cellular Component	GO:0000795	synaptonemal complex

InterPro / Pfam

InterPro	InterPro name
IPR000719	Protein kinase domain
IPR000959	POLO box domain
IPR008271	Serine/threonine-protein kinase, active site
IPR011009	Protein kinase-like domain superfamily
IPR017441	Protein kinase, ATP binding site
IPR033695	Second polo-box domain
IPR033701	First polo-box domain
IPR033702	Polo-like kinase 1, catalytic domain
IPR036947	POLO box domain superfamily

Pfam	Pfam name
PF00069	Protein kinase domain
PF00659	POLO box duplicated region

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-141444	Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-156711	Polo-like kinase mediated events	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-162658	Golgi Cisternae Pericentriolar Stack Reorganization	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-174178	APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-176412	Phosphorylation of the APC/C	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-176417	Phosphorylation of Emi1	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-2299718	Condensation of Prophase Chromosomes	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-2467813	Separation of Sister Chromatids	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-2500257	Resolution of Sister Chromatid Cohesion	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-2565942	Regulation of PLK1 Activity at G2/M Transition	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-2980767	Activation of NIMA Kinases NEK9, NEK6, NEK7	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-380259	Loss of Nlp from mitotic centrosomes	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-380270	Recruitment of mitotic centrosome proteins and complexes	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-380284	Loss of proteins required for interphase microtubule organization from the centrosome	Internal node	R-HSA-1640170	Cell Cycle
R-HSA-380320	Recruitment of NuMA to mitotic centrosomes	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-5620912	Anchoring of the basal body to the plasma membrane	Leaf	R-HSA-1852241	Organelle biogenesis and maintenance
R-HSA-5663220	RHO GTPases Activate Formins	Leaf	R-HSA-162582	Signal Transduction
R-HSA-68877	Mitotic Prometaphase	Internal node	R-HSA-1640170	Cell Cycle
R-HSA-68881	Mitotic Metaphase/Anaphase Transition	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-68884	Mitotic Telophase/Cytokinesis	Internal node	R-HSA-1640170	Cell Cycle
R-HSA-69273	Cyclin A/B1/B2 associated events during G2/M transition	Internal node	R-HSA-1640170	Cell Cycle
R-HSA-8852276	The role of GTSE1 in G2/M progression after G2 checkpoint	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-8854518	AURKA Activation by TPX2	Leaf	R-HSA-1640170	Cell Cycle
R-HSA-9648025	EML4 and NUDC in mitotic spindle formation	Leaf	R-HSA-1640170	Cell Cycle

Subcellular location

Location	ECO term	Pubmed
Chromosome, centromere, kinetochore
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome	ECO:0000269	PubMed:14654843
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome	ECO:0000269	PubMed:24018379
Cytoplasm, cytoskeleton, spindle	ECO:0000269	PubMed:18794143
Cytoplasm, cytoskeleton, spindle	ECO:0000269	PubMed:30715179
Midbody	ECO:0000269	PubMed:30715179
Nucleus

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Medium	Approved
appendix	glandular cells	Medium	Approved
bone marrow	hematopoietic cells	Low	Approved
breast	glandular cells	Medium	Approved
bronchus	respiratory epithelial cells	Medium	Approved
caudate	neuronal cells	Low	Approved
cerebellum	Purkinje cells	Low	Approved
cerebellum	cells in molecular layer	Low	Approved
cerebral cortex	neuronal cells	Low	Approved
cervix	glandular cells	Medium	Approved
cervix	squamous epithelial cells	Medium	Approved
colon	endothelial cells	Medium	Approved
colon	glandular cells	Medium	Approved
duodenum	glandular cells	Medium	Approved
endometrium 1	glandular cells	Low	Approved
epididymis	glandular cells	Low	Approved
esophagus	squamous epithelial cells	Medium	Approved
fallopian tube	glandular cells	Medium	Approved
gallbladder	glandular cells	Medium	Approved
heart muscle	cardiomyocytes	Medium	Approved
kidney	cells in glomeruli	Low	Approved
kidney	cells in tubules	Low	Approved
liver	hepatocytes	Medium	Approved
lung	alveolar cells	Low	Approved
lymph node	germinal center cells	Low	Approved
lymph node	non-germinal center cells	Low	Approved
nasopharynx	respiratory epithelial cells	Medium	Approved
oral mucosa	squamous epithelial cells	Medium	Approved
ovary	follicle cells	Low	Approved
ovary	ovarian stroma cells	Low	Approved
pancreas	exocrine glandular cells	Medium	Approved
placenta	trophoblastic cells	Medium	Approved
prostate	glandular cells	Medium	Approved
rectum	glandular cells	Medium	Approved
salivary gland	glandular cells	Low	Approved
seminal vesicle	glandular cells	Medium	Approved
skeletal muscle	myocytes	Medium	Approved
skin 1	Langerhans	Medium	Approved
skin 1	fibroblasts	Low	Approved
skin 1	keratinocytes	Medium	Approved
skin 1	melanocytes	Medium	Approved
skin 2	epidermal cells	Medium	Approved
small intestine	glandular cells	Medium	Approved
smooth muscle	smooth muscle cells	Medium	Approved
soft tissue 1	fibroblasts	Low	Approved
stomach 1	glandular cells	Low	Approved
stomach 2	glandular cells	Medium	Approved
testis	Leydig cells	High	Approved
testis	pachytene spermatocytes	High	Approved
testis	peritubular cells	High	Approved
testis	sertoli cells	High	Approved
thyroid gland	glandular cells	Low	Approved
tonsil	germinal center cells	Low	Approved
tonsil	non-germinal center cells	Low	Approved
tonsil	squamous epithelial cells	Medium	Approved
urinary bladder	urothelial cells	Medium	Approved
vagina	squamous epithelial cells	Medium	Approved

Summary of P53350

Gene: PLK1, PLK
Protein: Serine/threonine-protein kinase PLK1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P53350

Gene: PLK1, PLK Protein: Serine/threonine-protein kinase PLK1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: PLK1, PLK
Protein: Serine/threonine-protein kinase PLK1