MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.7.11.1	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; non-specific serine/threonine protein kinase
2.7.11.27	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; [acetyl-CoA carboxylase] kinase
2.7.11.31	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

Structure

PDB	Resolution (Å)	PDB name
2H6D	1.85	Protein Kinase Domain of the Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK alpha-2 chain)
2LTU		Solution Structure of autoinhibitory domain of human AMP-activated protein kinase catalytic subunit
2YZA	3.02	Crystal structure of kinase domain of Human 5'-AMP-activated protein kinase alpha-2 subunit mutant (T172D)
3AQV	2.08	Human AMP-activated protein kinase alpha 2 subunit kinase domain (T172D) complexed with compound C
4CFE	3.023	Structure of full length human AMPK in complex with a small molecule activator, a benzimidazole derivative (991)
4CFF	3.924	Structure of full length human AMPK in complex with a small molecule activator, a thienopyridone derivative (A-769662)
4ZHX	2.99	Novel binding site for allosteric activation of AMPK
5EZV	2.99	X-ray crystal structure of AMP-activated protein kinase alpha-2/alpha-1 RIM chimaera (alpha-2(1-347)/alpha-1(349-401)/alpha-2(397-end) beta-1 gamma-1) co-crystallized with C2 (5-(5-hydroxyl-isoxazol-3-yl)-furan-2-phosphonic acid)
5ISO	2.63	STRUCTURE OF FULL LENGTH HUMAN AMPK (NON-PHOSPHORYLATED AT T-LOOP) IN COMPLEX WITH A SMALL MOLECULE ACTIVATOR, A BENZIMIDAZOLE DERIVATIVE (991)
6B1U	2.77	Structure of full-length human AMPK (a2b1g1) in complex with a small molecule activator SC4
6B2E	3.8	Structure of full length human AMPK (a2b2g1) in complex with a small molecule activator SC4.
6BX6	2.9	AMP-Activated protein kinase (AMPK) inhibition by SBI-0206965: alpha 2 kinase domain bound to SBI-0206965
7MYJ	2.95	Structure of full length human AMPK (a2b1g1) in complex with a small molecule activator MSG011

GO term

GO ontology	GO term	GO description
Biological Process	GO:0016055	Wnt signaling pathway
Biological Process	GO:0006914	autophagy
Biological Process	GO:0071277	cellular response to calcium ion
Biological Process	GO:0042149	cellular response to glucose starvation
Biological Process	GO:0071333	cellular response to glucose stimulus
Biological Process	GO:0031669	cellular response to nutrient levels
Biological Process	GO:0034599	cellular response to oxidative stress
Biological Process	GO:0071380	cellular response to prostaglandin E stimulus
Biological Process	GO:0071466	cellular response to xenobiotic stimulus
Biological Process	GO:0006695	cholesterol biosynthetic process
Biological Process	GO:0097009	energy homeostasis
Biological Process	GO:0006633	fatty acid biosynthetic process
Biological Process	GO:0055089	fatty acid homeostasis
Biological Process	GO:0042593	glucose homeostasis
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0008610	lipid biosynthetic process
Biological Process	GO:1905691	lipid droplet disassembly
Biological Process	GO:0032007	negative regulation of TOR signaling
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0010629	negative regulation of gene expression
Biological Process	GO:1903944	negative regulation of hepatocyte apoptotic process
Biological Process	GO:1904428	negative regulation of tubulin deacetylation
Biological Process	GO:0010508	positive regulation of autophagy
Biological Process	GO:0045821	positive regulation of glycolytic process
Biological Process	GO:0016239	positive regulation of macroautophagy
Biological Process	GO:2000758	positive regulation of peptidyl-lysine acetylation
Biological Process	GO:1903829	positive regulation of protein localization
Biological Process	GO:1990044	protein localization to lipid droplet
Biological Process	GO:0006468	protein phosphorylation
Biological Process	GO:0042752	regulation of circadian rhythm
Biological Process	GO:0016241	regulation of macroautophagy
Biological Process	GO:0070507	regulation of microtubule cytoskeleton organization
Biological Process	GO:0062028	regulation of stress granule assembly
Biological Process	GO:0014850	response to muscle activity
Biological Process	GO:0048511	rhythmic process
Biological Process	GO:0007165	signal transduction
Molecular Function	GO:0004679	AMP-activated protein kinase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0050405	acetyl-CoA carboxylase kinase activity
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0004869	cysteine-type endopeptidase inhibitor activity
Molecular Function	GO:0140823	histone H2BS36 kinase activity
Molecular Function	GO:0047322	hydroxymethylglutaryl-CoA reductase (NADPH) kinase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0140677	molecular function activator activity
Molecular Function	GO:0004672	protein kinase activity
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Molecular Function	GO:0004712	protein serine/threonine/tyrosine kinase activity
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0030424	axon
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0030425	dendrite
Cellular Component	GO:0043025	neuronal cell body
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0031588	nucleotide-activated protein kinase complex
Cellular Component	GO:0005634	nucleus

InterPro / Pfam

InterPro	InterPro name
IPR000719	Protein kinase domain
IPR008271	Serine/threonine-protein kinase, active site
IPR011009	Protein kinase-like domain superfamily
IPR017441	Protein kinase, ATP binding site
IPR028375	KA1 domain/Ssp2, C-terminal
IPR028783	PRKAA2, UBA-like autoinhibitory domain
IPR032270	AMPK, C-terminal adenylate sensor domain
IPR039148	5'-AMP-activated protein kinase catalytic subunit alpha-2, C-terminal

Pfam	Pfam name
PF00069	Protein kinase domain
PF16579	Adenylate sensor of SNF1-like protein kinase

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1445148	Translocation of SLC2A4 (GLUT4) to the plasma membrane	Leaf	R-HSA-5653656	Vesicle-mediated transport
R-HSA-1632852	Macroautophagy	Internal node	R-HSA-9612973	Autophagy
R-HSA-163680	AMPK inhibits chREBP transcriptional activation activity	Leaf	R-HSA-1430728	Metabolism
R-HSA-200425	Carnitine metabolism	Leaf	R-HSA-1430728	Metabolism
R-HSA-2151209	Activation of PPARGC1A (PGC-1alpha) by phosphorylation	Leaf	R-HSA-1852241	Organelle biogenesis and maintenance
R-HSA-380972	Energy dependent regulation of mTOR by LKB1-AMPK	Leaf	R-HSA-162582	Signal Transduction
R-HSA-5628897	TP53 Regulates Metabolic Genes	Leaf	R-HSA-74160	Gene expression (Transcription)
R-HSA-6804756	Regulation of TP53 Activity through Phosphorylation	Leaf	R-HSA-74160	Gene expression (Transcription)
R-HSA-9613354	Lipophagy	Leaf	R-HSA-9612973	Autophagy
R-HSA-9619483	Activation of AMPK downstream of NMDARs	Leaf	R-HSA-112316	Neuronal System

Subcellular location

Location	ECO term	Pubmed
Cytoplasm	ECO:0000250
Nucleus	ECO:0000269	PubMed:15866171

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Low	Approved
appendix	glandular cells	Low	Approved
breast	glandular cells	Low	Approved
caudate	neuronal cells	Low	Approved
cerebellum	Purkinje cells	Low	Approved
cerebellum	cells in granular layer	Medium	Approved
cerebral cortex	neuronal cells	Low	Approved
cerebral cortex	neuropil	Medium	Approved
colon	glandular cells	Low	Approved
colon	peripheral nerve/ganglion	Low	Approved
endometrium 1	glandular cells	Low	Approved
endometrium 2	glandular cells	Low	Approved
epididymis	glandular cells	Low	Approved
fallopian tube	glandular cells	Medium	Approved
gallbladder	glandular cells	Low	Approved
heart muscle	cardiomyocytes	High	Approved
kidney	cells in glomeruli	Low	Approved
kidney	cells in tubules	High	Approved
lung	macrophages	Low	Approved
nasopharynx	respiratory epithelial cells	Low	Approved
ovary	follicle cells	Low	Approved
pancreas	pancreatic endocrine cells	Low	Approved
parathyroid gland	glandular cells	Low	Approved
rectum	glandular cells	Low	Approved
salivary gland	glandular cells	Low	Approved
seminal vesicle	glandular cells	Low	Approved
skeletal muscle	myocytes	High	Approved
soft tissue 2	chondrocytes	Low	Approved
stomach 1	glandular cells	Low	Approved
stomach 2	glandular cells	Low	Approved
testis	Leydig cells	Medium	Approved
testis	pachytene spermatocytes	Low	Approved
thyroid gland	glandular cells	Low	Approved
urinary bladder	urothelial cells	Low	Approved

Summary of P54646

Gene: PRKAA2, AMPK, AMPK2
Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-2

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of P54646

Gene: PRKAA2, AMPK, AMPK2 Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-2

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: PRKAA2, AMPK, AMPK2
Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-2