MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.7.11.1	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; non-specific serine/threonine protein kinase
2.7.11.26	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; tau-protein kinase
2.7.11.27	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; [acetyl-CoA carboxylase] kinase
2.7.11.31	Transferases; Transferring phosphorus-containing groups; Protein-serine/threonine kinases; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase

Structure

PDB	Resolution (Å)	PDB name
4RED	2.95	Crystal structure of human AMPK alpha1 KD-AID with K43A mutation
4RER	4.047	Crystal structure of the phosphorylated human alpha1 beta2 gamma1 holo-AMPK complex bound to AMP and cyclodextrin
4REW	4.58	Crystal structure of the non-phosphorylated human alpha1 beta2 gamma1 holo-AMPK complex
5EZV	2.99	X-ray crystal structure of AMP-activated protein kinase alpha-2/alpha-1 RIM chimaera (alpha-2(1-347)/alpha-1(349-401)/alpha-2(397-end) beta-1 gamma-1) co-crystallized with C2 (5-(5-hydroxyl-isoxazol-3-yl)-furan-2-phosphonic acid)
6C9F	2.924	AMP-activated protein kinase bound to pharmacological activator R734
6C9G	2.7	AMP-activated protein kinase bound to pharmacological activator R739
6C9H	2.65	non-phosphorylated AMP-activated protein kinase bound to pharmacological activator R734
6C9J	3.05	AMP-activated protein kinase bound to pharmacological activator R734
7JHG	3.47	Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Dorsomorphin (Compound C) and Fab-nanobody
7JHH	3.92	Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Fab and nanobody
7JIJ	5.5	ATP-bound AMP-activated protein kinase
7M74	3.93	ATP-bound AMP-activated protein kinase

GO term

GO ontology	GO term	GO description
Biological Process	GO:0061762	CAMKK-AMPK signaling cascade
Biological Process	GO:0016055	Wnt signaling pathway
Biological Process	GO:0006914	autophagy
Biological Process	GO:0015721	bile acid and bile salt transport
Biological Process	GO:0038183	bile acid signaling pathway
Biological Process	GO:0071277	cellular response to calcium ion
Biological Process	GO:0071361	cellular response to ethanol
Biological Process	GO:0042149	cellular response to glucose starvation
Biological Process	GO:0071333	cellular response to glucose stimulus
Biological Process	GO:0070301	cellular response to hydrogen peroxide
Biological Process	GO:0071456	cellular response to hypoxia
Biological Process	GO:0031669	cellular response to nutrient levels
Biological Process	GO:0071417	cellular response to organonitrogen compound
Biological Process	GO:0034599	cellular response to oxidative stress
Biological Process	GO:0071380	cellular response to prostaglandin E stimulus
Biological Process	GO:0071466	cellular response to xenobiotic stimulus
Biological Process	GO:0006695	cholesterol biosynthetic process
Biological Process	GO:0009631	cold acclimation
Biological Process	GO:0097009	energy homeostasis
Biological Process	GO:0006633	fatty acid biosynthetic process
Biological Process	GO:0055089	fatty acid homeostasis
Biological Process	GO:0019395	fatty acid oxidation
Biological Process	GO:0042593	glucose homeostasis
Biological Process	GO:0006006	glucose metabolic process
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0008610	lipid biosynthetic process
Biological Process	GO:1905691	lipid droplet disassembly
Biological Process	GO:0061744	motor behavior
Biological Process	GO:0032007	negative regulation of TOR signaling
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0010629	negative regulation of gene expression
Biological Process	GO:0046318	negative regulation of glucosylceramide biosynthetic process
Biological Process	GO:1903944	negative regulation of hepatocyte apoptotic process
Biological Process	GO:0046627	negative regulation of insulin receptor signaling pathway
Biological Process	GO:0050995	negative regulation of lipid catabolic process
Biological Process	GO:1904428	negative regulation of tubulin deacetylation
Biological Process	GO:0070050	neuron cellular homeostasis
Biological Process	GO:0045893	positive regulation of DNA-templated transcription
Biological Process	GO:1904179	positive regulation of adipose tissue development
Biological Process	GO:0010508	positive regulation of autophagy
Biological Process	GO:0008284	positive regulation of cell population proliferation
Biological Process	GO:0045542	positive regulation of cholesterol biosynthetic process
Biological Process	GO:0010628	positive regulation of gene expression
Biological Process	GO:0045821	positive regulation of glycolytic process
Biological Process	GO:1903109	positive regulation of mitochondrial transcription
Biological Process	GO:2000758	positive regulation of peptidyl-lysine acetylation
Biological Process	GO:1903829	positive regulation of protein localization
Biological Process	GO:1903955	positive regulation of protein targeting to mitochondrion
Biological Process	GO:0048643	positive regulation of skeletal muscle tissue development
Biological Process	GO:1990044	protein localization to lipid droplet
Biological Process	GO:0006468	protein phosphorylation
Biological Process	GO:0120188	regulation of bile acid secretion
Biological Process	GO:0042752	regulation of circadian rhythm
Biological Process	GO:0070507	regulation of microtubule cytoskeleton organization
Biological Process	GO:0033135	regulation of peptidyl-serine phosphorylation
Biological Process	GO:0062028	regulation of stress granule assembly
Biological Process	GO:0060627	regulation of vesicle-mediated transport
Biological Process	GO:0009411	response to UV
Biological Process	GO:0014823	response to activity
Biological Process	GO:0031000	response to caffeine
Biological Process	GO:0010332	response to gamma radiation
Biological Process	GO:0001666	response to hypoxia
Biological Process	GO:0048511	rhythmic process
Biological Process	GO:0007165	signal transduction
Molecular Function	GO:0004679	AMP-activated protein kinase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0050405	acetyl-CoA carboxylase kinase activity
Molecular Function	GO:0004691	cAMP-dependent protein kinase activity
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0004869	cysteine-type endopeptidase inhibitor activity
Molecular Function	GO:0140823	histone H2BS36 kinase activity
Molecular Function	GO:0047322	hydroxymethylglutaryl-CoA reductase (NADPH) kinase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0140677	molecular function activator activity
Molecular Function	GO:0004672	protein kinase activity
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Molecular Function	GO:0044877	protein-containing complex binding
Molecular Function	GO:0048156	tau protein binding
Molecular Function	GO:0050321	tau-protein kinase activity
Cellular Component	GO:0016324	apical plasma membrane
Cellular Component	GO:0030424	axon
Cellular Component	GO:0000785	chromatin
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0030425	dendrite
Cellular Component	GO:0043025	neuronal cell body
Cellular Component	GO:0016607	nuclear speck
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0031588	nucleotide-activated protein kinase complex
Cellular Component	GO:0005634	nucleus

InterPro / Pfam

InterPro	InterPro name
IPR000719	Protein kinase domain
IPR008271	Serine/threonine-protein kinase, active site
IPR011009	Protein kinase-like domain superfamily
IPR017441	Protein kinase, ATP binding site
IPR028375	KA1 domain/Ssp2, C-terminal
IPR028797	PRKAA1, UBA-like autoinhibitory domain
IPR032270	AMPK, C-terminal adenylate sensor domain
IPR039137	5'-AMP-activated protein kinase alpha 1 catalytic subunit, C-terminal

Pfam	Pfam name
PF00069	Protein kinase domain
PF16579	Adenylate sensor of SNF1-like protein kinase

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1632852	Macroautophagy	Internal node	R-HSA-9612973	Autophagy
R-HSA-380972	Energy dependent regulation of mTOR by LKB1-AMPK	Leaf	R-HSA-162582	Signal Transduction
R-HSA-5628897	TP53 Regulates Metabolic Genes	Leaf	R-HSA-74160	Gene expression (Transcription)
R-HSA-6804756	Regulation of TP53 Activity through Phosphorylation	Leaf	R-HSA-74160	Gene expression (Transcription)
R-HSA-9619483	Activation of AMPK downstream of NMDARs	Leaf	R-HSA-112316	Neuronal System

Subcellular location

Location	ECO term	Pubmed
Cytoplasm	ECO:0000269	PubMed:15866171
Nucleus	ECO:0000269	PubMed:15866171

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Medium	Supported
appendix	glandular cells	Medium	Supported
appendix	lymphoid tissue	Low	Supported
bronchus	respiratory epithelial cells	Medium	Supported
cerebral cortex	neuropil	Low	Supported
cervix	glandular cells	Low	Supported
colon	glandular cells	Medium	Supported
duodenum	glandular cells	Medium	Supported
endometrium 1	glandular cells	Low	Supported
epididymis	glandular cells	Medium	Supported
fallopian tube	glandular cells	Low	Supported
gallbladder	glandular cells	Medium	Supported
kidney	cells in tubules	Low	Supported
liver	hepatocytes	Low	Supported
nasopharynx	respiratory epithelial cells	Medium	Supported
oral mucosa	squamous epithelial cells	Low	Supported
pancreas	exocrine glandular cells	Low	Supported
placenta	decidual cells	Medium	Supported
placenta	trophoblastic cells	Low	Supported
prostate	glandular cells	Low	Supported
rectum	glandular cells	Medium	Supported
salivary gland	glandular cells	Low	Supported
skeletal muscle	myocytes	Medium	Supported
skin 2	epidermal cells	Low	Supported
small intestine	glandular cells	Medium	Supported
spleen	cells in red pulp	Medium	Supported
stomach 1	glandular cells	Medium	Supported
stomach 2	glandular cells	Medium	Supported
testis	Leydig cells	Medium	Supported
testis	sertoli cells	Medium	Supported
tonsil	non-germinal center cells	Low	Supported
tonsil	squamous epithelial cells	Low	Supported
urinary bladder	urothelial cells	Medium	Supported

Summary of Q13131

Gene: PRKAA1, AMPK1
Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of Q13131

Gene: PRKAA1, AMPK1 Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-1

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: PRKAA1, AMPK1
Protein: 5'-AMP-activated protein kinase catalytic subunit alpha-1