MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
2.3.2.27	Transferases; Acyltransferases; Aminoacyltransferases; RING-type E3 ubiquitin transferase
6.3.2.3	Ligases; Forming carbon-nitrogen bonds; Acid—amino-acid ligases (peptide synthases); glutathione synthase

Structure

PDB	Resolution (Å)	PDB name
4CFG	2.8	Structure of the TRIM25 coiled-coil
4LTB	2.59	Coiled-coil domain of TRIM25
5EYA	2.4	TRIM25 RING domain in complex with Ubc13-Ub conjugate
5FER	2.34	Complex of TRIM25 RING with UbcH5-Ub
5NT1	2.82	Complex of influenza A NS1 effector domain with TRIM25 coiled coil
5NT2	4.259	Complex of influenza A NS1 with TRIM25 coiled coil domain
6FLM	2.009	Crystal structure of the human TRIM25 PRYSPRY domain
6FLN	3.6	Crystal structure of the human TRIM25 coiled-coil and PRYSPRY domains

GO term

GO ontology	GO term	GO description
Biological Process	GO:0140374	antiviral innate immune response
Biological Process	GO:1990830	cellular response to leukemia inhibitory factor
Biological Process	GO:0002753	cytosolic pattern recognition receptor signaling pathway
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0046597	negative regulation of viral entry into host cell
Biological Process	GO:0051091	positive regulation of DNA-binding transcription factor activity
Biological Process	GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
Biological Process	GO:0051092	positive regulation of NF-kappaB transcription factor activity
Biological Process	GO:0070936	protein K48-linked ubiquitination
Biological Process	GO:0006513	protein monoubiquitination
Biological Process	GO:0032880	regulation of protein localization
Biological Process	GO:0046596	regulation of viral entry into host cell
Biological Process	GO:0043627	response to estrogen
Biological Process	GO:0006979	response to oxidative stress
Biological Process	GO:0033280	response to vitamin D
Biological Process	GO:0044790	suppression of viral release by host
Biological Process	GO:0030433	ubiquitin-dependent ERAD pathway
Biological Process	GO:0006511	ubiquitin-dependent protein catabolic process
Biological Process	GO:0019076	viral release from host cell
Molecular Function	GO:0039552	RIG-I binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0016874	ligase activity
Molecular Function	GO:0046872	metal ion binding
Molecular Function	GO:0140678	molecular function inhibitor activity
Molecular Function	GO:0003713	transcription coactivator activity
Molecular Function	GO:0061630	ubiquitin protein ligase activity
Molecular Function	GO:0004842	ubiquitin-protein transferase activity
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016604	nuclear body
Cellular Component	GO:0005654	nucleoplasm

InterPro / Pfam

InterPro	InterPro name
IPR001841	Zinc finger, RING-type
IPR001870	B30.2/SPRY domain
IPR003877	SPRY domain
IPR003879	Butyrophylin-like, SPRY domain
IPR006574	SPRY-associated
IPR013083	Zinc finger, RING/FYVE/PHD-type
IPR013320	Concanavalin A-like lectin/glucanase domain superfamily
IPR017907	Zinc finger, RING-type, conserved site
IPR027370	Zinc finger, RING-type, eukaryotic
IPR042753	TRIM25, PRY/SPRY domain
IPR043136	B30.2/SPRY domain superfamily

Pfam	Pfam name
PF00622	SPRY domain
PF13445	RING-type zinc-finger
PF13765	SPRY-associated domain

Reactome

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1169408	ISG15 antiviral mechanism	Leaf	R-HSA-168256	Immune System
R-HSA-168928	DDX58/IFIH1-mediated induction of interferon-alpha/beta	Internal node	R-HSA-168256	Immune System
R-HSA-5656169	Termination of translesion DNA synthesis	Leaf	R-HSA-73894	DNA Repair
R-HSA-5689896	Ovarian tumor domain proteases	Leaf	R-HSA-392499	Metabolism of proteins
R-HSA-877300	Interferon gamma signaling	Internal node	R-HSA-168256	Immune System
R-HSA-918233	TRAF3-dependent IRF activation pathway	Leaf	R-HSA-168256	Immune System
R-HSA-933541	TRAF6 mediated IRF7 activation	Leaf	R-HSA-168256	Immune System
R-HSA-933542	TRAF6 mediated NF-kB activation	Leaf	R-HSA-168256	Immune System
R-HSA-933543	NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10	Leaf	R-HSA-168256	Immune System
R-HSA-936440	Negative regulators of DDX58/IFIH1 signaling	Leaf	R-HSA-168256	Immune System
R-HSA-9692916	SARS-CoV-1 activates/modulates innate immune responses	Leaf	R-HSA-1643685	Disease
R-HSA-9705671	SARS-CoV-2 activates/modulates innate and adaptive immune responses	Leaf	R-HSA-1643685	Disease

Subcellular location

Location	ECO term	Pubmed
Cytoplasm	ECO:0000269	PubMed:17392790
Cytoplasm, Stress granule	ECO:0000269	PubMed:23950712
Nucleus	ECO:0000250

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Medium	Approved
appendix	glandular cells	Medium	Approved
bone marrow	hematopoietic cells	High	Approved
breast	glandular cells	High	Approved
breast	myoepithelial cells	Low	Approved
bronchus	respiratory epithelial cells	High	Approved
caudate	glial cells	Low	Approved
cerebellum	Purkinje cells	Low	Approved
cerebral cortex	neuronal cells	Medium	Approved
colon	endothelial cells	High	Approved
colon	glandular cells	High	Approved
colon	peripheral nerve/ganglion	Low	Approved
duodenum	glandular cells	High	Approved
endometrium 1	cells in endometrial stroma	Low	Approved
endometrium 1	glandular cells	High	Approved
endometrium 2	cells in endometrial stroma	Low	Approved
endometrium 2	glandular cells	High	Approved
epididymis	glandular cells	Medium	Approved
esophagus	squamous epithelial cells	High	Approved
fallopian tube	glandular cells	High	Approved
gallbladder	glandular cells	High	Approved
hippocampus	neuronal cells	Low	Approved
kidney	cells in tubules	Low	Approved
lung	macrophages	Medium	Approved
lymph node	germinal center cells	Medium	Approved
nasopharynx	respiratory epithelial cells	High	Approved
oral mucosa	squamous epithelial cells	High	Approved
ovary	ovarian stroma cells	Medium	Approved
pancreas	exocrine glandular cells	High	Approved
pancreas	pancreatic endocrine cells	Medium	Approved
parathyroid gland	glandular cells	Low	Approved
placenta	trophoblastic cells	High	Approved
prostate	glandular cells	Medium	Approved
rectum	glandular cells	High	Approved
salivary gland	glandular cells	Low	Approved
skin 1	Langerhans	Low	Approved
skin 1	fibroblasts	Medium	Approved
skin 1	keratinocytes	High	Approved
skin 1	melanocytes	High	Approved
skin 2	epidermal cells	High	Approved
small intestine	glandular cells	High	Approved
soft tissue 1	chondrocytes	Medium	Approved
soft tissue 1	fibroblasts	High	Approved
soft tissue 2	fibroblasts	Medium	Approved
spleen	cells in red pulp	Medium	Approved
spleen	cells in white pulp	Medium	Approved
stomach 1	glandular cells	High	Approved
stomach 2	glandular cells	High	Approved
testis	Leydig cells	Medium	Approved
testis	sertoli cells	Low	Approved
thyroid gland	glandular cells	Medium	Approved
tonsil	germinal center cells	Medium	Approved
tonsil	non-germinal center cells	Low	Approved
tonsil	squamous epithelial cells	High	Approved
urinary bladder	urothelial cells	High	Approved
vagina	squamous epithelial cells	Medium	Approved

Disease

Summary of Q14258

Gene: TRIM25, EFP, RNF147, ZNF147
Protein: E3 ubiquitin/ISG15 ligase TRIM25

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Summary of Q14258

Gene: TRIM25, EFP, RNF147, ZNF147 Protein: E3 ubiquitin/ISG15 ligase TRIM25

Structure

GO term

InterPro / Pfam

Reactome

Subcellular location

Interactor | Variant | Drug

Phenotype

Tissue and cell type

Disease

Gene: TRIM25, EFP, RNF147, ZNF147
Protein: E3 ubiquitin/ISG15 ligase TRIM25