MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
3.5.1.-	Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In linear amides;
3.5.1.23	Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In linear amides; ceramidase

PDB	Resolution (Å)	PDB name
4WGK	2.582	Crystal structure of human neutral ceramidase with Zn-bound phosphate

GO ontology	GO term	GO description
Biological Process	GO:0006915	apoptotic process
Biological Process	GO:0071345	cellular response to cytokine stimulus
Biological Process	GO:0046513	ceramide biosynthetic process
Biological Process	GO:0046514	ceramide catabolic process
Biological Process	GO:0006672	ceramide metabolic process
Biological Process	GO:0044241	lipid digestion
Biological Process	GO:0042759	long-chain fatty acid biosynthetic process
Biological Process	GO:2001234	negative regulation of apoptotic signaling pathway
Biological Process	GO:0007346	regulation of mitotic cell cycle
Biological Process	GO:0046512	sphingosine biosynthetic process
Biological Process	GO:0006670	sphingosine metabolic process
Molecular Function	GO:0017040	N-acylsphingosine amidohydrolase activity
Molecular Function	GO:0005509	calcium ion binding
Molecular Function	GO:0102121	ceramidase activity
Molecular Function	GO:0008270	zinc ion binding
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0005901	caveola
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005576	extracellular region
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005886	plasma membrane

InterPro	InterPro name
IPR006823	Neutral/alkaline nonlysosomal ceramidase
IPR031329	Neutral/alkaline non-lysosomal ceramidase, N-terminal
IPR031331	Neutral/alkaline non-lysosomal ceramidase, C-terminal
IPR038445	Neutral ceramidase, C-terminal domain superfamily

Pfam	Pfam name
PF04734	Neutral/alkaline non-lysosomal ceramidase, N-terminal
PF17048	Neutral/alkaline non-lysosomal ceramidase, C-terminal

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1660662	Glycosphingolipid metabolism	Leaf	R-HSA-1430728	Metabolism

Location	ECO term	Pubmed
Cell membrane	ECO:0000269	PubMed:15845354
Golgi apparatus membrane	ECO:0000269	PubMed:30154232
Membrane raft	ECO:0000250
Membrane, caveola	ECO:0000250
Mitochondrion	ECO:0000269	PubMed:10781606
Secreted	ECO:0000250
Secreted, extracellular exosome	ECO:0000269	PubMed:24798654

Tissue	Cell type	Expression level	Reliability
appendix	enterocytes	Low	Supported
colon	endocrine cells	Low	Supported
colon	endothelial cells	Low	Supported
colon	enterocytes	Low	Supported
duodenum	glandular cells	High	Supported
rectum	endothelial cells	High	Supported
rectum	enterocytes	Low	Supported
small intestine	endocrine cells	Medium	Supported
small intestine	enterocytes	Medium	Supported
small intestine	enterocytes - Microvilli	High	Supported