MultifacetedProtDB - Bologna Biocomputing Group

EC number	EC number description
3.4.22.-	Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases;

PDB	Resolution (Å)	PDB name
2CY7	1.9	The crystal structure of human Atg4B
2D1I	2.0	Structure of human Atg4b
2Z0D	1.9	The crystal structure of human Atg4B- LC3(1-120) complex
2Z0E	1.9	The crystal structure of human Atg4B- LC3(1-124) complex
2ZZP	2.05	The crystal structure of human Atg4B(C74S)- LC3(1-124) complex
5LXH	1.58	GABARAP-L1 ATG4B LIR Complex
5LXI	1.44	GABARAP-L1 ATG4B LIR Complex

GO ontology	GO term	GO description
Biological Process	GO:0006914	autophagy
Biological Process	GO:0016237	lysosomal microautophagy
Biological Process	GO:0016236	macroautophagy
Biological Process	GO:0000423	mitophagy
Biological Process	GO:0031173	otolith mineralization completed early in development
Biological Process	GO:0051697	protein delipidation
Biological Process	GO:0034497	protein localization to phagophore assembly site
Biological Process	GO:0015031	protein transport
Biological Process	GO:0006508	proteolysis
Biological Process	GO:0061912	selective autophagy
Molecular Function	GO:0019786	Atg8-specific peptidase activity
Molecular Function	GO:0004197	cysteine-type endopeptidase activity
Molecular Function	GO:0008234	cysteine-type peptidase activity
Molecular Function	GO:0004175	endopeptidase activity
Molecular Function	GO:0097110	scaffold protein binding
Cellular Component	GO:0005776	autophagosome
Cellular Component	GO:0031410	cytoplasmic vesicle
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005739	mitochondrion

InterPro	InterPro name
IPR005078	Peptidase C54
IPR038765	Papain-like cysteine peptidase superfamily
IPR046792	Peptidase C54, catalytic domain
IPR046793	Cysteine protease ATG4, F-type LIR motif

Pfam	Pfam name
PF03416	Peptidase family C54
PF20166	ATG4, F-type LIR motif

Reactome	Reactome Name	Node type	Reactome Root	Reactome Root Name
R-HSA-1632852	Macroautophagy	Internal node	R-HSA-9612973	Autophagy

Location	ECO term	Pubmed
Cytoplasm	ECO:0000269	PubMed:29165041
Cytoplasm, cytosol	ECO:0000269	PubMed:29165041
Cytoplasmic vesicle, autophagosome	ECO:0000269	PubMed:29165041
Endoplasmic reticulum	ECO:0000269	PubMed:29165041
Mitochondrion	ECO:0000269	PubMed:29165041

Tissue	Cell type	Expression level	Reliability
adrenal gland	glandular cells	Low	Approved
appendix	glandular cells	High	Approved
bone marrow	hematopoietic cells	Low	Approved
breast	glandular cells	Low	Approved
bronchus	respiratory epithelial cells	Low	Approved
cerebral cortex	neuronal cells	Low	Approved
colon	glandular cells	High	Approved
colon	peripheral nerve/ganglion	Medium	Approved
duodenum	glandular cells	High	Approved
fallopian tube	glandular cells	Low	Approved
gallbladder	glandular cells	Low	Approved
hippocampus	neuronal cells	Medium	Approved
kidney	cells in tubules	Low	Approved
lung	alveolar cells	Low	Approved
lung	macrophages	Medium	Approved
lymph node	non-germinal center cells	Medium	Approved
pancreas	exocrine glandular cells	Medium	Approved
placenta	trophoblastic cells	Medium	Approved
rectum	glandular cells	Medium	Approved
seminal vesicle	glandular cells	Low	Approved
skeletal muscle	myocytes	Low	Approved
small intestine	glandular cells	Medium	Approved
stomach 1	glandular cells	Medium	Approved
stomach 2	glandular cells	Low	Approved
thyroid gland	glandular cells	Low	Approved
tonsil	germinal center cells	Low	Approved
tonsil	non-germinal center cells	Low	Approved
urinary bladder	urothelial cells	Low	Approved
vagina	squamous epithelial cells	Low	Approved